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Ginsenoside Rb1 is Transformed into Rd and Rh2 by Microbacterium trichothecenolyticum
Ginsenoside Rb1 is Transformed into Rd and Rh2 by Microbacterium trichothecenolyticum
Journal of Microbiology and Biotechnology. 2013. Dec, 23(12): 1802-1805
Copyright © 2013, The Korean Society For Microbiology And Biotechnology
  • Received : July 18, 2013
  • Accepted : September 05, 2013
  • Published : December 28, 2013
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About the Authors
Hansoo Kim
Medical Proteomics Research Center, KRIBB, Daejeon 305-806, Republic of Korea
Jeong-Hoon Kim
Medical Proteomics Research Center, KRIBB, Daejeon 305-806, Republic of Korea
Phil Young Lee
Medical Proteomics Research Center, KRIBB, Daejeon 305-806, Republic of Korea
Kwang-Hee Bae
Medical Proteomics Research Center, KRIBB, Daejeon 305-806, Republic of Korea
Sayeon Cho
Department of Pharmacy, Chung-Ang University, Seoul 156-756, Republic of Korea
Byoung Chul Park
Medical Proteomics Research Center, KRIBB, Daejeon 305-806, Republic of Korea
Heungsop Shin
Department of Chemical Engineering and Biotechnology, Korea Polytechnic University, Gyeonggi-do 429-793, Republic of Korea
Sung Goo Park
Medical Proteomics Research Center, KRIBB, Daejeon 305-806, Republic of Korea
sgpark@kribb.re.kr

Abstract
Ginsenosides are the most important ingredient of ginseng and are known to possess many pharmacological and biological effects. Rb1, a major protopanaxadiol ginsenoside, is the most abundant ginsenoside in Panax ginseng C.A Meyer and can be hydrolyzed into more pharmaceutically potent minor ginsenosides. To identify a microorganism that is capable of converting Rb1 into other ginsenosides, we screened 12 Microbacterium spp., and M. trichothecenolyticum was identified as a likely candidate. M. trichothecenolyticum converted Rb1 into Rd and then into Rh2 based on TLC and HPLC analyses of reaction products. This biotransformation method can be easily applied for mass production of Rd and Rh2 by using Rb1.
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