Genomic Organization, Tissue Distribution and Developmental Expression of Glyceraldehyde 3-Phosphate Dehydrogenase Isoforms in Mud Loach Misgurnus mizolepis
Genomic Organization, Tissue Distribution and Developmental Expression of Glyceraldehyde 3-Phosphate Dehydrogenase Isoforms in Mud Loach Misgurnus mizolepis
Fisheries and aquatic sciences. 2013. Dec, 16(4): 291-301
Copyright ©2013, The Korean Society of Fisheries and Aquatic Science
This is an Open Access article distributed under the terms ofthe Creative Commons Attribution Non-Commercial Licens( whichpermits unrestricted non-commercial use, distribution, and reproduction inany medium, provided the original work is properly cited.
  • Received : July 11, 2013
  • Accepted : September 17, 2013
  • Published : December 30, 2013
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Sang Yoon, Lee
Dong Soo, Kim
Yoon Kwon, Nam

The genomic organization, tissue distribution, and developmental expression of two paralogous GAPDH isoforms were characterized in the mud loach Misgurnus mizolepis (Cypriniformes). The mud loach gapdh isoform genes ( mlgapdh-1 and mlgapdh-2 ) had different exon-intron organizations: 12 exons in mlgapdh-1 (spanning to 4.88 kb) and 11 in mlgapdh-2 (11.78 kb), including a non-translated exon 1 in each isoform. Southern blot hybridization suggested that the mud loach might possess the two copies of mlgapdh-1 and a single copy of mlgapdh-2 . The mlgapdh-1 transcript levels are high in tissues requiring high energy flow, such as skeletal muscle and heart, whereas mlgapdh-2 is expressed abundantly in the brain. Both isoforms are differentially regulated during embryonic and larval development, during which their expression is upregulated with the progress of development. Lipopolysaccharide challenge preferentially induced mlgapdh-2 transcripts in the liver. Therefore, the two isoforms have diversified functionally; mlgapdh-1 is associated more closely with energy metabolism, while mlgapdh-2 is related more to stress/immune responses, in the mud loach.
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH; EC is an essential, glycolytic enzyme to convert glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate, and this catalytic pathway for energy production is highly conserved in most aerobic organisms. However, in addition to the housekeeping role of GAPDH in the carbohydrate metabolism, a number of studies have already demonstrated that mammalian GAPDHs should be closely involved in diverse nonglycolytic cellular pathways such as stress response, apoptosis and neurodegenerative disorders (Sirover, 2005; Tristan et al., 2011).
Both mammals and teleosts express two functional forms of GAPDHs (GAPDH-1 and GAPDH-2), and molecular phylogenetic studies have suggested undoubtedly that both isoforms of teleostean GAPDHs reveal the potential orthology to their corresponding counterparts of mammalian GAPDH isoforms (Kim and Nam, 2008; Sarropoulou et al., 2011). However, previous studies have also highlighted that teleost GAPDH-2 might have a different evolutionary history from that of mammalian GAPDH-2, and also that the physiological roles of teleostean GAPDH-2 might have been diversified from that common to mammalian orthologue (Manchado et al., 2007; Cho et al., 2008). For example, teleost GAPDH-2 lacks the Pro-rich N-terminal peptide that is typical of mammalian GAPDH-2 and, unlike mammalian GAPDH-2 showing the spermatogenic cell-specific expression (Bunch et al., 1998; Welch et al., 2000, 2006), transcripts of teleostean GAPDH-2 are found in a wide array of tissues (Cho et al., 2008).
Recently, teleost GAPDHs have also been begun to be reported for their potential involvements in nonglycolytic functions. They include the modulatory roles in the innate immunity (Cho et al., 2008, 2011) and embryonic development (Manchado et al., 2007; Sarropoulou et al., 2011). However, in contrast to rich information on mammalian GAPDHs, the functional analysis of fish GAPDHs has been still limited, in which the expression characteristics in the nonglycolytic pathways have been exemplified in only a few species as above. Moreover, at the genomic levels, the information on organizational features of both gapdh isoforms in teleost genomes has been insufficient so far.
In the present study, we describe the genomic organization of the two gapdh genes exploited from the mud loach ( Misgurnus mizolepis ; named gapdh-1 and mlgapdh-2 ), a commercially important, aquaculture-relevant freshwater species in Korea, and scrutinized the patterns for tissue- and developmental stage-dependent expression of the both gapdh isoforms. In addition, the acute immune response of the two gapdh isoforms at mRNA levels was also examined by using the lipopolysaccharide (LPS) challenge.
Oligonucleotide primers and thermal cycling conditions used in this studyA predenaturation step at 94℃ for 2 min was prepared before each PCR reaction.RT, room temperature; qPCR, quantitative PCR.
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Oligonucleotide primers and thermal cycling conditions used in this study A predenaturation step at 94℃ for 2 min was prepared before each PCR reaction. RT, room temperature; qPCR, quantitative PCR.
Materials and Methods
- Genomic cloning and sequence analysis
Genomic sequences of the gapdh-1 and mlgapdh-2 genes were isolated using Expand High Fidelity PCR System (Roche Applied Science, Manheim, Germany). Primer pairs (mlGAPDH1 FW/RV for gapdh-1 and mlGAPDH2 FW/RV for mlgapdh-2 that had been designated based on the cDNA sequence of each isoform were used for PCR isolation. Information on the oligonucleotide primers and the thermal cycling conditions used in this study is provided in Table 1.The amplified products were TA-cloned and at least nine PCR clones were sequenced for each isoform at both directions. To compare the genomic organizations of mlgapdh isoforms with their potential orthologues, genomic gapdhs sequences published in literatures or public databases were compiled. Also we deciphered gapdh genomic sequences from other teleost genomes available in Ensembl Genome Browser ( Blast and text searches were carried out. Because the Ensembl Genome Database often contains unfinished draft regarding sequence annotations and assemblies, we edited and/or re-annotated the exon-intron organization for each gapdh genomic sequence based on the comparison with transcribed sequence, which is available in either Ensembl, other databases or published literatures.
- Southern blot hybridization analysis
Genomic Southern blot hybridization was used to examine the organization and presence patterns of the mlgapdh-1 and mlgapdh-2 isoforms in the mud loach genome. Dra II-digested 5 μg genomic DNA was separated on a 1% agarose gel, transferred to nylon membrane with the capillary transfer method, and hybridized with a digoxygenin-labeled probe. The genomic region targeted by each probe (labeled probes A, B, and C) is shown in Fig. 3 . All procedures, including probe labeling, hybridization, stringent washing, and signal detection, were performed with the DIG DNA Labeling and Detection Kit (Roche Applied Science, Mannheim, Germany), according to the manufacturer’s manual.
- Biological samples for mRNA expression assays
First, to examine the tissue-specific expression of each mlgapdh isoform, a quantitative real-time reverse transcription (RT)-PCR assay was conducted with 12 tissues (brain, eye, fin, gill, heart, intestine, kidney, liver, muscle, spleen, ovary, and testis). The tissue samples were obtained surgically from healthy six-month-old individuals ( n = 16; mean body weight [BW] = 19.6 ± 4.8 g). Tissue samples from the 16 individuals (eight females and eight males) were subjected to total RNA extraction for the quantitative PCR (qPCR) assays.
Second, to examine the expression patterns of the mlgapdh transcripts in developing embryos and early larvae, the pooled eggs from two females were artificially fertilized with sperm from three males, according to the procedure described by Kim et al. (1994). The fertilized embryos were placed in an incubator at 24 ± 0.5℃ and approximately 300 embryos were sampled at 0 (just after fertilization), 2 (32-cell stage), 4 (early-blastula stage), 6 (early-gastrula stage), 8 (late-gastrula stage), 12 (3-4-myotome stage), 16 (16-17-myotome stage), 20 (23-24-myotome stage), and 24 (tail beating) h post-fertilization (HPF). After hatching (28 HPF; day 0), about 110 larvae were also sampled at 1, 2, 3 and 4 days posthatching (DPH) for RNA preparation. The embryogenesis and early ontogenesis of the mud loach were described in detail by Kim et al. (1987).
Third, to examine the potential differential expression of the mud loach gapdh genes in response to endotoxin exposure, we performed LPS ( Escherichia coli 0111:B4; Sigma-Aldrich, St Louis, MO, USA) challenge. Six mud loaches (average BW = 16 ± 4 g) were given intraperitoneal injections of LPS (suspended in phosphate-buffered saline [pH 7.4] at a dose of 0, 2, or 5 μg/g BW). The injected fish were transferred to one of three replicate rectangular cages (40 × 60 × 30 cm 3 = W × D × H) installed in a large tank (2 × 4 × 1 m 3 = W × D × H) containing 500 L of tap water at 25 ± 1.0℃. After 24 h, the liver was removed from each fish and subjected to gene expression assays.
- Real-time qPCR assay ofgapdhtranscripts
Total RNA was purified using the RNeasy Midi Kit (Qiagen, Hilden, Germany), including the DNase I treatment step, according to the manufacturer’s protocol. Total RNA (2 μg) was reverse transcribed using the Qiagen Omniscript Reverse Transcription System (Qiagen), using an oligo-d(T)20 primer (1 μM final concentration) and a mud loach 18S rRNA primer (ML18S-RV; 0.05 M final concentration) (Nam et al., 2011). The reaction conditions were according to the manufacturer’s protocol. The RT product (cDNA sample) was diluted twofold (for gapdh cDNAs) or tenfold (for 18S rRNA) with sterile distilled water and then the diluted cDNA sample (1 μL) was used as the template for the thermal cycling reaction. Standard curves for gapdh-1 , mlgapdh-2 , and 18S rRNA were prepared with five log-dilutions of each positive plasmid sample, and PCR efficiencies of> 90% were confirmed before the assays. Real-time PCR amplification (25 μL reaction volume) was performed using the 2× iQ™ SYBR Green Supermix (Bio-Rad, Hercules, CA, USA). The PCR primer pairs were mlGAPDH1 q1F/1R (amplicon, 263 bp), mlGAPDH2 q1F/1R (amplicon, 273 bp), and qML18S 1F/1R (amplicon, 274 bp) for gapdh-1 , mlgapdh-2 , and 18S rRNA, respectively. The threshold cycle number (Ct) used was the default setting in the iCycler iQ™ Real-Time PCR Detection System (Bio-Rad). To determine the basal expression levels of the mlgapdh isoforms in adult tissues (including LPS-stimulated tissues) and developing embryos/larvae, the expression of each mlgapdh isoform in each sample was normalized to the 18S rRNA level of the same sample. The relative expression levels were calculated according to a method described previously (Schmittgen and Livak, 2008). All assays were performed in triplicate. Differences in the relative expression levels among samples were assessed by analysis of variance (ANOVA) followed by Duncan’s multiple range test with a significance level of P = 0.05, using the SPSS software ver. 10.1.3 (SPSS Inc., Chicago, IL, USA).
Results and Discussion
- Gene structure and organization of mud loach gapdh isoforms
The two mlgapdh isoform genes have different genomic structures. Based on a comparative analysis of the cDNA (JN230712 for gapdh-1 and JN230713 for mlgapdh-2 )
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Gene structure and organization of gapdh orthologues and paralogues from teleosts. Horizontal solid lines are introns, while exons (E) are shown as boxes. Non-translated exon 1s (NTE1s) are indicated by blank boxes and coding regions by filled boxes. Dashed horizontal lines are used when the intron lengths are inaccurate due to large numbers of ambiguous base calling in the database. Accession code or reference for each gapdh sequence is indicated at the right side.
and genomic sequences, mlgapdh-1 (JN230716) has 11 translated exons (exons 2-12) and one non-translated exon (exon 1; NTE1). The NTE1 (25 bp) of mlgapdh-1 begins 43 bp after the putative TATA signal and the length of intron 1 is 366 bp. In the first translated exon (exon 2), the ATG translation initiation site occurs 23 bp from the 5′ end of exon 2. Intron 2 (between exons 2 and 3 of mlgapdh-1 ) is significantly larger (2,426 bp) than the other introns. In contrast, mlgapdh-2 (JN230717) has 10 translated exons (exons 2-11) and an NTE1 (21 bp). Intron 1 (between NTE1 and exon 2) in mlgapdh-2 was markedly longer (6,321 bp) than in mlgapdh-1 (366 bp). The intron that interrupts exons 3 and 4 of mlgapdh-2 was significantly longer (1,175 bp) than that in mlgapdh-1 (83 bp). Based on a pairwise alignment of the two isoforms, exon 9 of mlgapdh-2 is separated in mlgapdh-1 (exon 9 and exon 10). Except for this exon separation (the conversion of exons 9 and 10 in mlgapdh-1 into exon 9 in mlgapdh-2 ), the numbers of amino acids encoded by the other exons were similar in mlgapdh-1 and mlgapdh-2 (Appendix 1).
From bioinformatics mining, we detected two copies of gapdh-1 in several teleost species such as the Atlantic cod Gadus morhua , stickleback Gasterosteus aculeatus and Nile tilapia Oreochromis niloticus , whereas only one copy of gapdh-2 was seen in all fish species searched ( Fig. 1 ). Comparisons of the genomic organizations of the gapdh isoforms among teleosts indicated that fish share conserved genomic organization features for both isoforms, although the lengths of non-coding regions vary markedly. In terms of similarly, many teleostean gapdh genes possessed non-translated exons based on our bioinformatics analyses. Although non-translated exons have not been clearly identified in several fish gapdh genes, due to incomplete sequence information in the database, they likely also have a non-translated exon. The numbers of amino acids encoded by each gapdh gene were largely homogenous regardless of fish species. However, further extension of the comparisons with other representative vertebrate orthologs (fish, human, mouse, rat, and chicken) showed that the exon-intron organization varied among species, although they encoded similar total numbers of amino acids (333-351). The number of exons varies among those species (seven in mouse to 12 in fish and chicken), and several of the exons seen in the lower vertebrates have merged into fewer but larger exons in mammals ( Fig. 2 ). This suggests that vertebrate gapdh-1 genes might have evolved from a common ancestor through exon shuffling or rearrangement.
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Schematic diagrams to show exon-intron organization of gapdh genes from representative vertebrates. Two teleost species (mud loach andzebrafish) were selected for comparison with other vertebrates. Horizontal lines are the flanking regions and introns (not drawn to scale), while exons(E) are shown as boxes. Coding regions are filled with colors while non-coding regions and non-translated exon 1s (NTE1s) are in blank. Numerical figureprovided for each translated exon is the length (bp) of coding nucleotide sequence. Translation start site (ATG) and stop codon are indicated by verticalarrows. Accession code and the number of amino acids encoded for each gapdh sequence are indicated at the right side. In the comparison amonggapdh-1 orthologues, the three exons 3, 4, and 5 of human, chicken and fish gapdh-1s are merged into either two (exons 3 and 4 in rat) or one (exon 3 inmouse) larger exon(s) in murine gapdh-1s. As similarly, two exons 6 and 7 in human, chicken and fish gapdh-1s appear in a larger, merged exon in murinegapdh-1s (exon for mouse and exon 5 for rat). On the other hand, the largest exon of human gapdh-1 (exon 8) is divided into multiple smaller exons inother orthologues (two exons for murine gapdh-1s [exons 4 and 5 for mouse and exons 6 and 7 for rat], and four exons 8-10 for chicken and fish gapdh-1s).However such an exon rearrangement (i.e., merge/separation) is not observable between gapdh-2 (or gapdh-s) orthologues.
Unlike the gapdh-1 genes, the mammalian and fish gapdh-2 genes have the same numbers of exons (11 exons), although there are significant differences in the deduced lengths of the polypeptides of the mammalian and teleost GAPDH-2 proteins because of the long proline-rich N-terminal domain in the mammalian GAPDH-2 proteins. However in contrast to mammalian gapdh-2 genes, the fish gapdh-2 genes may have an untranslated exon, although a non-translated exon-1 has not been found in all teleost gapdh genes, likely due to the errors in sequencing and/or annotation in the Ensembl database. The identification of the NTE1 in teleost gapdhs was not highlighted yet in a recent study of two perciform species (Sarropoulou et al., 2011). Despite this difference, the mammalian and teleost gapdh-2 genes have identical or similar numbers of nucleotides (and amino acids encoded) in each of the remaining exons (exons 3-11) ( Fig. 2 ). No distinct genomic isoform of gapdh-2 has been identified in the chicken, although adult chickens are known to express diverse transcript variants of gapdh , including alternatively spliced and alternatively initiated forms (Mezquita et al., 1998). Together, these data suggest that evolutionary divergence patterns of the two gapdh paralogs differ among the various taxonomic groups.
- Southern blot hybridization pattern
Genomic Southern blot hybridization patterns were polymorphic for both gapdh-1 and mlgapdh-2 in six unrelated individuals ( Fig. 3 ). The first blot of gapdh-1 probed with probe-A produced two hybridization bands for all six individuals, and four different hybridization patterns were observed (hybridization bands at approximately> 14.0/7.0 kb, 9.5/7.0 kb, 9.0/7.0 kb, and 7.2/9.2 kb). The second blot for gapdh-1 probed with probe-B showed the same hybridization pattern as probe-A. In contrast, the hybridization analysis of mlgapdh-2 using probe-C showed that four of the six individuals displayed only one hybridization signal (a ~9.0-kb band for fishes #1, #4, and #6, and a 9.5-kb band for fish #2), whereas the other two individuals (fishes #3 and #5) displayed two hybridization bands (at 8.0 and 9.0 kb).
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Southern blot analysis of mlgapdh isoforms with DraII-digested genomic DNA from six unrelated mud loach individuals are hybridized with probe-A, -B or -C. Relative positions of DraII-restriction sites and probe binding regions are also provided at the top.
If each of gapdh genes existed as a single copy in the mud loach genome, we would expect a Southern blot hybridization of DraII-digested genomic DNA to show a single band larger than 5.4 kb (for gapdh-1 ) or 5.1 kb (for mlgapdh-2 ). However, two hybridization bands were detected in all six individuals (for gapdh-1 ), and in two of the six individuals (for mlgapdh-2 ). Because each fish showed similar hybridization intensity between the two bands for gapdh-1 , the present result suggests of the presence of one additional gapdh-1 copy in the mud loach genome, as observed in several other teleosts (Sarropoulou et al., 2011) (see also Fig. 1 ). The size differences in the two hybridization bands among individuals (particularly for fish #1) could result from polymorphism in the Dra II-restriction sites among individuals; further analysis with a larger number of fish samples is needed to further explore this explanation. However, for mlgapdh-2 , the hybridization pattern was not identical to that of gapdh-1 , in terms of the signal intensities of the two hybridization bands in any given individual (for example, fish #3 or #5 in the blot), which were much weaker than the signal from the single hybridization band of fish #1, #2, #4, or #6. This suggests that polymorphism in mlgapdh-2 may have resulted in allelic differences in the restriction sites recognized by Dra II, rather than from variability in the gene copy numbers among individuals. This would also be consistent with our bioinformatics analysis, showing only one copy of gapdh-2 in all teleost species examined. Together, our Southern blot results suggest that the mud loach possesses two paralogous copies ( i.e ., subisoforms) of gapdh-1 and one of gapdh-2 in its genome and also that both isoforms may represent an allelic difference in the restriction sites of DraII, depending on the individuals. It is also known that mammalian genomes often contain multiple pseudogene copies (50-364 copies) of duplicated gapdh genes, which have been acquired through retrotranspositional activity (Garcia-Meunier et al., 1993; Liu et al., 2009). Currently, it is unclear whether the additional copy of gapdh-1 undiscovered yet is functional.
- Tissue distribution and expression levels of mlgapdh transcripts
As expected, the mRNAs of both mlgapdh isoforms were detected ubiquitously in all tissues examined, but the basal mRNA levels were variable among tissues and between isoforms ( Fig. 4 ). The gapdh-1 transcripts were present predominantly in skeletal and heart muscles ( P <0.05).
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Tissue distribution patterns and basal expression levels of mudloach gapdh isoform transcripts as assessed by qPCR analysis based onthe normalization against 18S rRNA expression. Tissues examined are thebrain (Br), eye (Ey), fin (Fi), gill (Gi), heart (He), intestine (In), kidney (Ki),liver (Li), muscle (Mu), spleen (Sp), ovary (Ov), and testis (Te). Mean ± SDs(from triplicate assays) with different letters were significantly differentbased on ANOVA followed by Duncan’s multiple ranged tests at P = 0.05.
The ovary also contained higher amounts of gapdh-1 transcripts versus other tissues ( P <0.05). The liver, gills, and intestine showed moderate levels of mlgapdh-1 transcripts, whereas other tissues examined showed weak or minimal expression. In contrast, the highest levels of mlgapdh-2 transcripts were found in the brain, followed by eye (P<0.05). The other tissues examined, including the gill, kidney, spleen, testis, and fin, showed moderate levels of mlgapdh-2 transcripts, whereas the heart and intestine harbored low levels ( P <0.05). The tissues such as ovary, liver, and skeletal muscle contained very low levels of mlgapdh-2 transcripts ( P <0.05). From a comparison of the basal mRNA levels of mlgapdh-1 and mlgapdh-2 in specific tissues, the transcript levels of mlgapdh-1 in muscle and ovary were up to 450-fold higher than those of mlgapdh-2 ( P <0.05). The mlgapdh-1 transcript levels were also 40 and 23 fold higher than the mlgapdh-2 levels in the liver and heart, respectively (P<0.05). Conversely, the expression of mlgapdh-2 was significantly higher than that of mlgapdh-1 in the brain, eye, fin, spleen, and testis ( P <0.05).
The ubiquitous detection in all tissues examined is consistent with the protein’s essential housekeeping roles in cellular energy metabolism. However, the basal expression levels of the two isoforms were variable among tissues. The mlgapdh-1 mRNA was strongly predominant in muscle tissues (skeletal muscle and heart), as is mammalian gapdh-1 (Welch et al., 2000). The tissue distribution patterns observed in this study were similar to previous reports that gapdh-1 genes are strongly expressed in high-energy-requiring tissues, such as the skeletal muscle and heart, whereas the gapdh-2 genes are consistently most strongly expressed in the brain (Manchado et al., 2007; Cho et al., 2008). Although only three studies have quantitatively assayed gapdh isoforms among tissues, the expression patterns were fairly consistent in three distantly related species: rockbream ( Oplegnathus fasciatus , Perciformes) (Cho et al., 2008), Senegalese sole ( Solea senegalensis , Pleuronectiformes) (Manchado et al., 2007), and mud loach ( M. mizolepis , Cypriniformes) (this study). This suggests that the tissue-specific roles of the two GAPDH isoforms may be broadly conserved in the teleost lineage, although the number of fish species examined must be increased. The mud loach testis showed higher expression of mlgapdh-2 than mlgapdh-1 , consistent with previous findings for flatfish gapdh-2 (Manchado et al., 2007). However, the potential roles of the GAPDH-2 isoform in spermatogenesis and/or male fertility remain to be explored (Miki et al., 2004).
- Developmental expression of mlgapdh transcripts
Both gapdh isoforms were dynamically regulated in developing mud loach embryos and early larvae. Overall, the levels of mlgapdh-1 and mlgapdh-2 transcripts increased gradually with embryonic development, and then increased sharply from around hatching ( Fig. 5 ). The mRNA levels of gapdh-1 were relatively constant until 12 HPF, began to increase from 16 HPF, and then increased rapidly in subsequent stages ( P <0.05). At hatching (day 0), the amount of gapdh-1 transcripts was ~ 100 fold higher than the initial level in the fertilized eggs. Thereafter, the level remained constant until day 2 (2 DPH), and then increased slightly at days 3 and 4 ( P <0.05). In contrast, the mlgapdh-2 transcript levels decreased upon fertilization ( P <0.05) and were maintained at low levels until 12 HPF. Like mlgapdh-1 , the expression of mlgapdh-2 was initially activated at 16 HPF (P<0.05), and then increased continuously until 3 DPH (day 3), when they stabilized. During embryonic development, the expression of mlgapdh-1 transcripts was higher than that of mlgapdh-2 transcripts, regardless of the developmental stage. The ratios of mlgapdh-1 expression to mlgapdh-2 expression ranged from 2 (during D2-D4) to more than 270 (at 2 HPF), when the relative ratio decreased gradually with embryonic development and as early ontogenesis progressed, except at 0 and 12 HPF.
In this study, the relative levels of gapdh-1 transcript were always higher than that of mlgapdh-2 transcripts
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Developmental expression of two mud loach gapdh isoforms during embryogenesis (hours post fertilization; HPF) and early larval ontogenesis (days post hatching; DPH), as assessed by real-time quantitative PCR (qPCR) analysis based on the normalization against 18S rRNA expression. (A) Expression pattern of each gapdh isoform mRNA during embryonic and larval development. (B) Relative ratios of mlgaphd-1 to mlgapdh-2 transcripts at each developmental stage. Mean ± SDs (from triplicate assays) with different letters were significantly different based on ANOVA followed by Duncan’s multiple ranged tests at P = 0.05. For better views, expression levels during early embryogenesis up to 16 h HPF (in A) and relative ratios between isoforms at stages D0-D4 (in B) are presented again with finer scales of y-axis.
throughout embryogenesis and ontogenesis, suggesting that MLGAPDH-1 may be more closely associated with the early development and ontogeny of this species than MLGAPDH-2. The expression of the mlgapdh-1 isoform was initially upregulated from 16 HPF (corresponding to the heart-beating stage) (Kim et al., 1987), increased sharply at hatching, and was maintained thereafter at a relatively constant level. This finding is consistent with high energy demand during hatching and larval development, including during muscle differentiation.
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Hepatic expression of mud loach gapdh isoforms at mRNA levels in response to lipopolysaccharide (LPS) injection. Differential expression was assessed by real-time quantitative PCR (qPCR) analysis. Based on the normalization against 18S rRNA control, mean ± SDs (from triplicate assays) with different letters were significantly different based on ANOVA followed by Duncan’s multiple ranged tests at P = 0.05.
Manchado et al. (2007) also reported the differential regulation of the gapdh isoforms during the metamorphosis of flatfish larvae, in that the expression of gapdh-1 , but not gapdh-2 , was significantly modulated, perhaps in response to the energy demands during the metamorphic stage. In contrast, the activation of mlgapdh-2 during development occurs slightly later than that of mlgapdh-1 , and the increase in mlgapdh-2 is prominent only after 24 HPF, suggesting that the role of MLGAPDH-2 is important during the larval stage rather than in the early embryo. Meanwhile, the functional involvement of GAPDH (especially GAPDH-1) as a maternal gene with high expression levels in unfertilized eggs has also been reported in perciform species (Mommens et al., 2010; Sarropoulou et al., 2011).
- Modulation of hepatic mlgapdh transcript levels by LPS challenge
LPS injection upregulated the expression of both mlgapdh isoforms in the liver, but the patterns of upregulation differed ( Fig. 6 ). The mlgapdh-1 transcripts were induced only in fish injected with the higher dose (5 μg LPS/g BW; P <0.05) and the degree of induction (up to twofold) was less than that observed for mlgapdh-2 . In contrast, mlgapdh-2 transcripts were induced significantly in those tissues by both dose levels of LPS (2 μg and 5 μg LPS/g BW; P <0.05). Furthermore, the increase in mlgapdh-2 transcripts was dose-dependent (five- and ninefold after doses of 2 and 5 μg LPS/g BW, respectively, relative to that in non-challenged fish).
As shown in Fig. 6 , the inducibility and responsiveness of mlgapdh-2 were greater than those of mlgapdh-1 . Although few quantitative comparisons of the expression of the gapdh isoforms in response to a given stimulatory treatment have been made in fish, several studies of the utility of gapdh as an internal control for assessment of gene expression in fish have reported the possible modulation of gapdh transcripts by various stimulatory factors (Tang et al., 2007; Mitter et al., 2009; Dang and Sun, 2011). Such differential inducibility between isoforms could be related, at least in part, to the different patterns of transcription factor-binding motifs in their 5′-flanking regulatory regions. The 5′-flanking region of mlgapdh-2 contains more copies of several immunity- or stress-related motifs/elements than does mlgapdh-1 (Cho et al., 2011). Collectively, our results are consistent with previous reports of the modulated expression of gapdh in aquatic animals in response to bacterial and/or viral challenges (Cho et al., 2008; Booth and Bilodeau-Bourgeois, 2009), and with the upregulation of gapdh genes in a rat model of LPS-induced inflammation (Xie et al., 2006). Although we have not yet clarified the role(s) of inflammation-induced GAPDH proteins in the pathogenesis of infections in this species, LPS injection is known to cause severe inflammatory responses and tissue damage, triggering apoptosis (Gao and Kwaik, 2000; Bannerman and Goldblum, 2003; DeLeo, 2004; Li et al., 2005). Several previous studies of fish have also shown that LPS elicits typical apoptotic characteristics in vitro and in vivo (MacKenzie et al., 2006a, 2006b; Xiang et al., 2008).
In summary, two paralogous isoforms of the gapdh genes of the mud loach were isolated and characterized at the genomic level. Both mlgapdh-1 and mlgapdh-2 revealed the similar non-translated exons; however, the two isoforms have different exon-intron organizations. The mud loach apparently possesses two paralogous copies of mlgapdh-1 and a single mlgapdh-2 . Gapdh-1 transcripts predominate in tissues that require a high energy flow, while gapdh-2 is expressed abundantly in the brain. Both isoforms are differentially regulated during embryonic and larval development, during which mlgapdh-1 expression is always higher than that of mlgapdh-2 . In contrast, LPS challenge preferentially induced mlgapdh-2 , rather than mlgapdh-1 , transcripts in the liver. Therefore, mlgapdh-1 may be associated more closely with energy metabolism, whereas mlgapdh-2 is related more to stress/immune responses in the mud loach.
- Appendix 1.
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Genomic organization of two mud loach gapdh paralogues (gapdh-1 and mlgapdh-2) (A) and pairwise alignment of deduced amino acid sequences between MLGAPDH-1 and MLGAPHD-2 (B).The isoform gapdh-1 possesses 11 translated exons (E2-E12) and one non-translated exon (25-bp E1), while mlgapdh-2 represents 10 translated exons (E2-E11) and one non-translated exon (21-bp E1). Exon9 (54 aa) of mlgapdh-2 is separate in gapdh-1 (26-aa exon 9 and 28-aa exon 10).
This study was supported by a research grant of Pukyong National University (2013 Year)
Bannerman DD , Goldblum SE. 2003 Mechanisms of bacterial lipopolysaccharide-induced endothelial apoptosis. Am J Physiol Lung Cell Mol Physiol 284 L899 - L914    DOI : 10.1152/ajplung.00338.2002.
Booth NJ , Bilodeau-Bourgeois AL. 2009 Proteomic analysis of head kidney tissue from high and low susceptibility families of channel catfish following challenge with Edwardsiella ictaluri. Fish Shellfish Immunol 26 193 - 196    DOI : 10.1016/j.fsi.2008.03.003.
Bunch DO , Welch JE , Magyar PL , Eddy EM , O’Brien DA 1998 Glyceraldehyde 3-phosphate dehydrogenase-S protein distribution during mouse spermatogenesis. Biol Reprod 58 834 - 841    DOI : 10.1095/biolreprod58.3.834.
Cho YS , Lee SY , Kim KH , Nam YK. 2008 Differential modulations of two glyceraldehyde 3-phosphate dehydrogenase mRNAs in response to bacterial and viral challenges in a marine teleost Oplegnathus fasciatus (Perciformes). Fish Shellfish Immunol 25 472 - 476    DOI : 10.1016/j.fsi.2008.07.007.
Cho YS , Kim DS , Nam YK 2011 Isoform-specific response of two GAPDH paralogs during bacterial challenge and metal exposure in mud loach (Misgurnus mizolepis; Cypriniformes) kidney and spleen. J Fish Pathol 24 269 - 278    DOI : 10.7847/jfp.2011.24.3.269.
Dang W , Sun L. 2011 Determination of internal controls for quantitative real time RT-PCR analysis of the effect of Edwardsiella tarda infection on gene expression in turbot (Scophthalmus maximus). Fish Shellfish Immunol 30 720 - 728    DOI : 10.1016/j.fsi.2010.12.028.
DeLeo FR. 2004 Modulation of phagocyte apoptosis by bacterial pathogens. Apoptosis 9 399 - 413    DOI : 10.1023/B:APPT.0000031448.64969.fa.
Gao LY , Kwaik YA. 2000 The modulation of host cell apoptosis by intracellular bacterial pathogens. Trends Microbiol 8 306 - 313    DOI : 10.1016/S0966-842X(00)01784-4.
Garcia-Meunier P , Etienne-Julan M , Fort P , Piechaczyk M , Bonhomme F. 1993 Concerted evolution in the GAPDH family of retrotransposed pseudogenes. Mamm Genome 4 695 - 703    DOI : 10.1007/BF00357792.
Kim DS , Jo JY , Lee TY. 1994 Induction of triploidy in mud loach (Misgurnus mizolepis) and its effect on gonad development and growth. Aquaculture 120 263 - 270    DOI : 10.1016/0044-8486(94)90083-3.
Kim KY , Nam YK. 2008 Evolutionary history of two paralogous glyceraldehyde 3-phosphate dehydrogenase genes in teleosts. J Fish Sci Technol 11 177 - 181    DOI : 10.5657/fas.2008.11.3.177.
Kim YU , Park YS , Kim DS. 1987 Development of eggs, larvae and juveniles of loach, Misgurnus mizolepis Gunther. Bull Korean Fish Soc 20 16 - 23
Li T , Hu J , Thomas JA , Li L. 2005 Differential induction of apoptosis by LPS and taxol in monocytic cells. Mol Immunol 42 1049 - 1055    DOI : 10.1016/j.molimm.2004.09.032.
Liu YJ , Zheng D , Balasubramanian S , Carriero N , Khurana E , Robilotto R , Gerstein MB. 2009 Comprehensive analysis of the pseudogenes of glycolytic enzymes in vertebrates: the anomalously high number of GAPDH pseudogenes highlights a recent burst of retrotrans-positional activity. BMC Genomics 10 480 -    DOI : 10.1186/1471-2164-10-480.
MacKenzie S , Iliev D , Liarte C , Koskinen H , Planas JV , Goetz FW , Molsa H , Krasnov A , Tort L. 2006a Transcriptional analysis of LPS-stimulated activation of trout (Oncorhynchus mykiss) monocyte/macrophage cells in primary culture treated with cortisol. Mol Immunol 43 1340 - 1348    DOI : 10.1016/j.molimm.2005.09.005.
MacKenzie S , Montserrat N , Mas M , Acerete L , Tort L , Krasnov A , Goetz FW , Planas JV. 2006b Bacterial lipopolysaccharide induces apoptosis in the trout ovary. Reprod Biol Endocrinol 4 46 -    DOI : 10.1186/1477-7827-4-46.
Manchado M , Infante C , Asensio E , Canavate JP. 2007 Differential gene expression and dependence on thyroid hormones of two glyceraldehyde-3-phosphate dehydrogenases in the flatfish Senegalese sole (Solea senegalensis Kaup). Gene 400 1 - 8    DOI : 10.1016/j.gene.2007.05.008.
Mezquita J , Pau M , Mezquita C. 1998 Several novel transcripts of glyceraldehyde-3-phosphate dehydrogenase expressed in adult chicken testis. J Cell Biochem 71 127 - 139    DOI : 10.1002/(SICI)1097-4644(19981001)71:1<127::AID-JCB13>3.0.CO;2-K.
Miki K , Qu W , Goulding EH , Willis WD , Bunch DO , Strader LF , Perreault SD , Eddy EM , O’Brien DA. 2004 Glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme, is required for sperm motility and male fertility. Proc Natl Acad Sci U S A 101 16501 - 16506    DOI : 10.1073/pnas.0407708101.
Mitter K , Kotoulas G , Magoulas A , Mulero V , Sepulcre P , Figueras A , Novoa B , Sarropoulou E. 2009 Evaluation of candidate reference genes for QPCR during ontogenesis and of immune-relevant tissues of European seabass (Dicentrarchus labrax). Comp Biochem Physiol B Biochem Mol Biol 153 340 - 347    DOI : 10.1016/j.cbpb.2009.04.009.
Mommens M , Fernandes JM , Bizuayehu TT , Bolla SL , Johnston IA , Babiak I. 2010 Maternal gene expression in Atlantic halibut (Hippoglossus hippoglossus L.) and its relation to egg quality. BMC Res Notes 3 138 -    DOI : 10.1186/1756-0500-3-138.
Nam YK , Cho YS , Lee SY , Kim BS , Kim DS. 2011 Molecular characterization of hepcidin gene from mud loach (Misgurnus mizolepis; Cypriniformes). Fish Shellfish Immunol 31 1251 - 1258    DOI : 10.1016/j.fsi.2011.09.007.
Sarropoulou E , Nousdili D , Kotoulas G , Magoulas A. 2011 Functional divergences of GAPDH isoforms during early development in two perciform fish species. Mar Biotechnol 13 1115 - 1124    DOI : 10.1007/s10126-011-9375-6.
Schmittgen TD , Livak KJ. 2008 Analyzing real-time PCR data by the comparative CT method. Nat Protoc 3 1101 - 1108    DOI : 10.1038/nprot.2008.73.
Sirover MA 2005 New nuclear functions of the glycolytic protein, glyceraldehyde-3-phosphate dehydrogenase, in mammalian cells. J Cell Biochem 95 45 - 52    DOI : 10.1002/jcb.20399.
Tang R , Dodd A , Lai D , McNabb WC , Love DR. 2007 Validation of zebrafish (Danio rerio) reference genes for quantitative real-time RT-PCR normalization. Acta Biochim Biophys Sin 39 384 - 390    DOI : 10.1111/j.1745-7270.2007.00283.x.
Tristan C , Shahani N , Sedlak TW , Sawa A. 2011 The diverse functions of GAPDH: views from different subcellular compartments. Cell Signal 23 317 - 323    DOI : 10.1016/j.cellsig.2010.08.003.
Welch JE , Barbee RR , Magyar PL , Bunch DO , O’Brien DA. 2006 Expression of the spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase (GAPDS) in rat testis. Mol Reprod Dev 73 1052 - 1060    DOI : 10.1002/mrd.20235.
Welch JE , Brown PL , O’Brien DA , Magyar PL , Bunch DO , Mori C , Eddy EM. 2000 Human glyceraldehyde 3-phosphate dehydrogenase-2 gene is expressed specifically in spermatogenic cells. J Androl 21 328 - 338
Xiang LX , Peng B , Dong WR , Yang ZF , Shao JZ. 2008 Lipopolysaccharide induces apoptosis in Carassius auratus lymphocytes, a possible role in pathogenesis of bacterial infection in fish. Dev Comp Immunol 32 992 - 1001    DOI : 10.1016/j.dci.2008.01.009.
Xie W , Shao N , Ma X , Ling B , Wei Y , Ding Q , Yang G , Liu N , Wang H , Chen K 2006 Bacterial endotoxin lipopolysaccharide induces up-regulation of glyceraldehyde-3-phosphate dehydrogenase in rat liver and lungs. Life Sci 79 1820 - 1827    DOI : 10.1016/j.lfs.2006.06.018.