Two urostyloid ciliates, collected from brackish water in Korea, were identified as
Diaxonella pseudorubra pseudorubra
(Kaltenbach, 1960) Berger, 2006 and
Pseudokeronopsis flava
(Cohn, 1866) Wirnsberger, Larsen and Uhlig, 1987. The description was based on living, protargol impregnated specimens. These species are described as follows:
Diaxonella pseudorubra pseudorubra
: body size
in vivo
145-230 × 40-60 μm, elongated ellipsoidal in shape. Cytoplasm reddish and flexible. Adoral zone of membranelles occupied 30-40% of the body; composed of 33-44 membranelles; 1-3 frontoterminal cirri, 1-4 frontal row cirri, 4-6 buccal cirri, 6-10 transverse cirri. Midventral rows composed of 14-24 cirri, four left marginal rows, one right marginal row. Two kinds of cortical granules; the larger one is yellowish and the smaller one is reddish.
Pseudokeronopsis flava
: body size
in vivo
150-210 × 30-45 μm, elongated ellipsoidal shape. Cytoplasm yellowish and flexible. Adoral zone of membranelles occupied 25-30% of body; composed of 44-58 membranelles in number. Frontal cirri forming bicorona composed of 5-7 cirral pairs, 2-3 frontoterminal cirri, one buccal cirrus, and 2-3 transverse cirri. Midventral rows composed of 18-33 cirri, 34-53 left marginal cirri, and 40-58 right marginal cirri. Two kinds of cortical granules; the larger one is colorless and “blood-cell-shaped,” and the smaller one is yellowish
. Diaxonella pseudorubra pseudorubra
is different from the most similar subspecies,
D. pseudorubra pulchra
, in cytoplasmic color and number of midventral cirri.
Pseudokeronopsis flava
is different from its most similar congeners in pigment granular color, number of bicorona, number of midventral cirri, and position of the con-tractile vacuole.
INTRODUCTION
The urostyloid ciliates are composed of a large group of hy-potrichs,
sensu lato
, which belong to the superfamily Urosty-loidea, and more than 2,000 species have been described (Ber-ger, 2006; Lynn, 2008). Since the superfamily Urostyloidea was established by Bütschli (1889), its definition has been improved as usually having an ellipsoidal, middle to large sized body and a midventral complex between the marginal rows (Corliss, 1979; Lynn and Small, 2002; Berger, 2006;Lynn, 2008). The systematics of genus
Diaxonella
has a comparatively complex history, including many synonyms (Jankowski, 1979; Foissner, 1987; Oberschmidleitner and Aescht, 1996; Berger, 2006; Shao et al., 2007).
Diaxonella pseudorubra
(Kaltenbach, 1960) was a monotypic species of the genus
Diaxonella
, but three subspecies,
D. pseudoru-bra pseudorubra, D. pseudorubra polystylata,
and
D. pseudorubra pulchra
, have been recognized recently by Berger (2006). The genus Pseudokeronopsis includes 10 species and is usually found in marine environments (Song et al., 2002, 2004, 2006). Most
Pseudokernopsis
species have co-rtical granules colored red, yellow, or orange-yellow, which can be used to distinguish the species. In this study, we pro-vide morphological redescriptions and variations in two urostyloids from Korea.
MATERIALS AND METHODS
- Sample collection and enrichment
Diaxonella pseudorubra pseudorubra
and
Pseudokeronopsis flava
were collected from brackish waters in Korea.
Diaxo-nella pseudorubra pseudorubra
: (October, 2010; salinity 2‰) downstream of the sewage treatment plant (N34̊ 55′ 23′ ′, E128̊ 07′ 07′ ′) located in Sadeung-dong, Sacheon-si, Gyeong-sangnam-do. The water samples including the ciliates were collected with twigs, leaves, wood, and mud.
P. flava
: (Ja-nuary, 2010; salinity 20‰) in the Soesokkak estuary (N33̊ 15′ 7.19′′, E126̊ 37′ 24.59′′), Seogwipo-si, Jeju-do. The spe-cimens were transferred to a Petri dish (87 mm in diameter)or a cell culture dish (150 mm in diameter). These ciliates have been cultured at room temperature in the laboratory with collected water, enriched with dried wheat grain to supply fungal and bacterial nutrients
- Morphological observation
The morphology of living specimens was observed under low (50-400×) and high (1,000×; immersion oil) magnifi-cations using a light microscope with a DIC device (Axio Imager A1; Carl Zeiss, Oberkochen, Germany) and their images were captured using a CCD camera (Axio Cam MRc; Carl Zeiss). The infraciliatures were observed after impre-gnation using the protagol method (Wilbert, 1975; Foissner, 1992). Terminology and taxonomic classification are accord-
Morphometric data of Diaxonella pseudorubra pseudorubraMin, minimum; Max, maximum; Med, median; CV, coefficient of variation in %; n, population size; L, live specimens; S, stained specimens; AZM, adoralzone of membranelles; BC, buccal cirrus; FC, frontal cirri; FTC, frontoterminal cirri; FRC, frontal row cirri; TC, transverse cirri; PTC, pretransverse cirri; MVC, midventral cirri; RMC, right marginal cirri; LMC, left marginal cirri; DK, dorsal kineties.
Morphometric data of Diaxonella pseudorubra pseudorubra Min, minimum; Max, maximum; Med, median; CV, coefficient of variation in %; n, population size; L, live specimens; S, stained specimens; AZM, adoralzone of membranelles; BC, buccal cirrus; FC, frontal cirri; FTC, frontoterminal cirri; FRC, frontal row cirri; TC, transverse cirri; PTC, pretransverse cirri; MVC, midventral cirri; RMC, right marginal cirri; LMC, left marginal cirri; DK, dorsal kineties.
ing to Berger (2006) and Lynn (2008).
SYSTEMATIC ACCOUNTS
-
Order Urostylida Jankowski, 1979
-
Superfamily Urostyloidea Butschli, 1889
-
Family Holostichidae Faure-Fremiet, 1961
-
Genus1*DiaxonellaJankowski, 1979
-
2*Diaxonella pseudorubra pseudorubra(Kaltenbach, 1960)Tables 1,2,Figs. 1-3)
Keronopsis pseudorubra
Kaltenbach, 1960 (cited from Berger, 2006: 470).
Keronopsis rubra
Jerka-Dziadosz and Janus, 1972: 249.
Diaxonella trimarginata
Jankowski, 1979: 83; Oberschmi-dleitner and Aescht, 1996: 21; Shao et al., 2007: 25.
Holosticha pseudorubra
: Foissner, 1987: 225; Berger, 2001: 44.
Diaxonella pseudorubra pseudorubra
: Berger, 2006: 468.
Description. General morphology and behavior :
Body
Comparisons of Diaxonella pseudorubra pseudorubra which has different namesAll data are based on protargol-impregnated specimens. AMs, adoral membranelles; BC, buccal cirri; FC, frontal cirri; FTC, frontoterminal cirri; FRC, frontalrow cirri; TC, transverse cirri; MVC, midventral cirri; RMC, right marginal cirri; LMR, left marginal row; PTC, pretransverse ventral cirri; Mi, micronucleus; UM, undulating membranes; CV, contractile vacuole.
Comparisons of Diaxonella pseudorubra pseudorubra which has different names All data are based on protargol-impregnated specimens. AMs, adoral membranelles; BC, buccal cirri; FC, frontal cirri; FTC, frontoterminal cirri; FRC, frontalrow cirri; TC, transverse cirri; MVC, midventral cirri; RMC, right marginal cirri; LMR, left marginal row; PTC, pretransverse ventral cirri; Mi, micronucleus; UM, undulating membranes; CV, contractile vacuole.
Diaxonella pseudorubra pseudorubra from live (A-F) and impregnated specimens (G H). A Ventral view of a typical individual; B C Arrangement of cortical granules on ventral and dorsal sides; D Flattened lateral view; E Two kinds of cortical granules ondorsal side; F Various body shapes; G Somatic and oral infraciliature of ventral side; H Dorsal kineties and nuclear apparatus.AZM adoral zone of membranelles; BC buccal cirrus; DK dorsal kineties; EM endoral membrane; FC frontal cirri; FRC frontalrow cirri; FTC frontoterminal cirri; LMC left marginal cirri; Ma macronuclear nodules; Mi micronuclei; MVC midventral cirri; PM paroral membrane; PTC pretransverse cirri; RMC right marginal cirri; TC transverse cirri. Scale bars: A D G=50 μm E=5 μm.
Photomicrographs of Diaxonella pseudorubra pseudorubra from live specimens. A Ventral view of a typical individual; B Flattened lateral view; C Contractile vacuole (arrowhead); D Somatic ciliature of the ventral side; E Arrangement of corticalgranules on the ventral side (arrowheads); F Buccal cirri (arrows) and undulating membranes (arrowhead); G Patterns of corticalgranules: larger (arrowhead) and smaller (arrow) arrangement around dorsal bristle of smaller cortical granules (double arrowhead); H Dorsal bristles (arrowhead); I Pretransverse cirri (arrow) and transverse cirri (arrowhead); J-L Various food vacuoles; J Diatom Pleurosigma sp.; K Aspidisca sp.; L Small particle of wheat grain. Scale bars: A=50 μm G H=5 μm.
size 145-230 × 40-60 μm, usually about 170× 40 μm, length: width ratio about 4 : 1 on average in live specimens. Body shape elongated and ellipsoid with rounding at both ends(
Figs.1
A,
2
A,
3
A), dorsoventrally flattened about 2 : 1, ven-tral side slightly concave, dorsal side convex (
Figs.1
D,
2
B). Cytoplasm reddish to wine color and flexible (
Fig.2
D). Sin-gle contractile vacuole spherical and above the mid-body near the left margin (
Figs.1
A,
2
C). Locomotion usually crawl-ing on substrate. Omnivorous feeding (
Fig.2
J-L).
Buccal field and oral infraciliature:
Adoral zone of mem-branelles occupies 30-40% of body length (
Figs.1
G,
3
A), distal to proximal continuously semicircular, consists of 33-44 adoral membranelles (
Fig.3
B), widest membranelle about 10 μm in length. Buccal area narrow and rather deep. Undu-lating membranes intersecting to half of the membranes, endoral membrane slightly curved and longer, paroral mem-brane anteriorly curved and shorter, distal end of paroral beyond endoral, about 45 μm in total length (
Figs.1
G,
3
B).
Somatic infraciliature:
Usually, four frontal cirri slightly enlarged, lying on front of the anterior region but one frontal cirrus near the distal end of the adoral zone of membranelles(
Figs.1
G, 3C), about 17μm long. One to three frontoterminal cirri located on the right side of frontal cirri, about 12μm long (
Fig.3
D). Four to six buccal cirri arranged along paroral membrane, about 8 μm long (
Figs.2
F,
3
B). Inconspicuous, usually four frontal row cirri starting at the same level at the distal end of the paroral membrane and terminating at the same level at intersecting point of the undulating membranes(
Figs.1
G,
3
E). Midventral complex composed of 14-24 pairs of midventral cirri, continued frontoterminal cirri, arranged in a zigzag pattern, terminating at the posterior half but most cirri separated from others (
Figs.1
G,
3
G). Six to ten trans-verse cirri arranged in a J-shape, about 18 μm long (
Figs.2
I,
3
H). Two pretransverse cirri located near right transverse cirrus (
Fig.3
H). One right marginal row consisting of 30-50 cirri (
Figs.1
G,
3
G). Four left marginal rows that gradually
Photomicrographs of Diaxonella pseudorubra pseudorubra from impregnated specimens. A Ventral view of a typical individual; B Buccal cirri (arrowhead) and undulating membranes (arrow); C Four frontal cirri (arrowheads); D Two frontoterminal cirri (arro-wheads); E Frontal row cirri (arrowheads); F Four left marginal rows (arrowheads); G Right marginal row (arrowhead) and midventralcomplex (arrow); H Pretransverse cirri (arrow) and transverse cirri (arrowhead); I Dorsal kineties (arrowheads) and extra dorsalbristles (arrow); J Macronuclear (arrowhead) and micronuclei (arrows). Scale bars: A=50 μm J=10 μm.
shorten from right to left composed of 21-38, 20-33, 18-28,and 4-15 cirri, respectively (
Figs.1
G,
3
F). Three dorsal kine-ties complete, but some cases of extra dorsal bristles present,bristle about 3-4 μm in length (
Figs.1
H,
2
H,
3
I).
Cortical granules:
Two kinds of cortical granules present on both sides and pigmented: larger one greenish yellow about 1 μm in diameter, arranged linearly in short groups beside the cirral rows on the ventral side and densely arranged on the dorsal side but loosely around each kineties (
Fig.1
B, C). Smaller one red wine colored, about 0.3μm in diameter, scat-tered around the whole body and arranged linearly in short groups between larger granules or around each cirrus (
Figs.1
E,
2
G).
Nuclear apparatus:
Various shaped which elongated ellip-soidal to ovoid macronuclear nodules throughout the whole body, about 120 in number with several nucleoli. Spherical shaped micronuclei scattered body and 2.5 μm in diameter(
Figs.1
H,
3
A, J).
Distribution.
Europe (Austria, Germany, and Poland), Africa (Burundi) and Asia (China, Korea [present study]).
Remarks.
The taxonomy of the genus
Diaxonella
is relatively complicated. According to a review by Berger (2006) on this subspecies, Kaltenbach (see Berger, 2006) described original population that had two frontal rows, a reddish body color, several buccal cirri, and one left marginal row. Berger (2006) considered
Keronopsis pseudorubra
sensu Kaltenbach in 1960 as a senior synonym of
Diaxonella
trimarginata sensu Jankowski (1979) which was renamed as a new species and new genus based on
Keronopsis rubra
sensu Jerka-Dziadosz and Janus (1972), because they misidentified it. Furthermore, he changed the name from
Keronopsis pseudorubra
to
Diax-onella pseudorubra
. Berger (2006) also suggested that the genus
Diaxonella
is monotypic and divided
D. pseudorubra
into three subspecies, which are distinguished by body color, number of midventral pairs, and habitat. However, Shao et al. (2007) did not mention this suggestion of Berger (2006), because their publications overlapped in time.
Consequently, this Korean population of
Diaxonella pseu-dorubra pseudorubra
agrees with the original description except in the number of left marginal rows (4 vs. 1) and frontal cirri (3-5 vs. forming bicorona), which were intensively considered by Berger (2006). Additionally, our population agrees well with subsequent redescriptions of Austrian, Chi-nese, and Polish populations (Jerka-Dziadosz and Janus, 1972; Jankowski, 1979; Oberschmidleitner and Aescht, 1996; Shao et al., 2007). The Korean population differs slightly from the Austrian population of
D. pseudorubra pseudorubra
in shape of micronuclei (spherical vs. ellipsoidal), number of frontal cirri (3-5 vs. 2-3), canals in the contractile vacuole (absent vs. present), number of pretransverse cirri (2 vs. 3-4), and position of the last midventral cirrus (posterior half vs. pos-terior) (Oberschmidleitner and Aescht, 1996). Furthermore, the Korean population differs from the Chinese population at the intersecting point (middle vs. posterior) of the undulat-ing membranes, the arrangement of reddish small cortical granules (linearly grouped vs. sparsely scattered), and the shape and diameter of micronuclei (spherical, about 2.5 μm vs. ovoid, about 5 μm) (Shao et al., 2007). Moreover, several populations (Europe, Asia, and Africa) of
D. pseudorubra pseudorubra
have been found in freshwater but this Korean population was recovered from brackish water of an estuarine
Morphometric data of Pseudokeronopsis flavaMin, minimum; Max, maximum; Med, median; CV, coefficient of variation in %; n, population size; L, live specimens; S, stained specimens; AZM, adoral zone of membranelles; FC, frontal cirri; Bicorona, FC arranged in arcs forming cirri; BC, buccal cirrus; FTC, frontoterminal cirri; TC, transversecirri; MVC, midventral cirri; LMC, left marginal cirri; RMC, right marginal cirri; DK, dorsal kineties.
Morphometric data of Pseudokeronopsis flava Min, minimum; Max, maximum; Med, median; CV, coefficient of variation in %; n, population size; L, live specimens; S, stained specimens; AZM, adoral zone of membranelles; FC, frontal cirri; Bicorona, FC arranged in arcs forming cirri; BC, buccal cirrus; FTC, frontoterminal cirri; TC, transversecirri; MVC, midventral cirri; LMC, left marginal cirri; RMC, right marginal cirri; DK, dorsal kineties.
littoral zone (Berger, 2006).
-
Family Pseudokeronopsidae Borror & Wicklow, 1983
-
Genus Pseudokeronopsis Borror & Wicklow, 1983
-
1*Pseudokeronopsis flava(Cohn, 1866) (Tables 3, 4, Figs. 4-6)
Oxytricha flava
Cohn, 1866: 288.
Holosticha flava
Kent, 1882: 769.
Keronopsis rubra
Kahl, 1932: 571.
Pseudokeronopsis flava
: Wirnsberger et al., 1987: 79; Berger, 2001: 55; Song et al., 2004: 1137; Sun and Song, 2005:81; Berger, 2006: 940; Song et al., 2006: 272.
Description. General morphology and behavior:
Body size 150-210× 30-45 μm, usually about 180× 40 μm, length : width ratio about 5 : 1 in live specimens. Body shape elon-gated elliptical, both ends narrowly rounded, anterior portion usually concave leftwards (
Figs.4
A,
5
A), dorsoventrally flat-tened about 2 : 1 (
Fig.5
B). One contractile vacuole located below the mid-body near the left cell margin about 13 μm in diameter (
Figs.4
A,
5
E). Cytoplasm very flexible but not con-tractile(
Fig.5
C), almost yellowish at low magnification. Locomotion usually crawling on substrate, wrapping to change direction (
Fig.5
C). Omnivorous feeding (
Fig.5
J, K).
Buccal field and oral infraciliature:
Adoral zone of mem-branelles occupies 25-30% of body length, composed of 44-
Comparisons of different populations of Pseudokeronopsis flavaAll data are based on protargol-impregnated specimens. AMs, adoral membranelles; MVC, midventral cirri; TC, transverse cirri; UM, undulating membranes; EM, endoral membrane; PM, paroral membrane.
Comparisons of different populations of Pseudokeronopsis flava All data are based on protargol-impregnated specimens. AMs, adoral membranelles; MVC, midventral cirri; TC, transverse cirri; UM, undulating membranes; EM, endoral membrane; PM, paroral membrane.
Pseudokeronopsis flava from live (A-E) and impregnated specimens (F G). A Ventral view of a typical individual; B Arrange-ment of cortical granules on the ventral side; C Three cortical granular rows on the dorsal side; D E Cortical granule groups (arrowhead) and “blood-cell-shaped” granules (arrow) apparatus; F Somatic and oral infraciliature of the ventral side; G Threedorsal kineties and nuclear apparatus. AZM adoral zone of membranelles; BC buccal cirrus; Bicorona FC arranged in arcs formingcirri; DK dorsal kineties; EM endoral membrane; FTC frontoterminal cirri; LMC left marginal cirri; Ma macronuclear nodules; MVCmidventral cirri; PM paroral membrane; RMC right marginal cirri; TC transverse cirri. Scale bars: A G=50 μm E=2 μm.
58 adoral membranelles (
Figs.4
F,
6
A). Buccal area narrow and rather deep. Paroral and endoral membranes slightly curved, intersecting to half of the membranes, endoral longer than paroral, about 35 μm in length (
Figs.4
F,
6
A, D).
Somatic infraciliature:
Frontal cirri arranged in two arcs forming bicorona comprising 5-7 cirral pairs, bicorona con-nect to midventral complex (
Fig.6
E). Midventral complex extended to subposterior and consisting of 18-33 cirri; distance between posterior most cirrus of midventral cirri and upper-most transverse cirrus about 20 μm (
Figs.4
F,
6
A). Two or
Photomicrographs of Pseudokeronopsis flava from live specimens. A Ventral view of a typical individual; B Flattened lateralview; C Showing flexible body; D Dorsal kineties (arrowheads); E Contractile vacuole (arrowhead); F Arrangement of corticalgranule on ventral side; G H Cortical granule groups (arrowheads) and “blood-cell-shaped” granules (arrow) apparatus on dorsalside; I Three transverse cirri (arrowhead); J K Various food vacuoles; J One kind of Chlorophyta; K Coleps sp. Scale bar: A=50 μm.
three frontoterminal cirri located at the right side of the right-most bicorona pair (
Fig.6
C, E). Buccal cirrus located near the intersecting point of the undulating membranes (
Fig.6
D).Two to four transverse cirri located posteriorly and distinctly separated by marginal rows (
Figs.5
I,
6
F). Right marginal row commenced at level of the down most bicorona cirrus,terminated at the posterior part and comprising 40-58 cirri (
Figs.4
F,
6
A, E), left marginal row with 34-53 cirri; posterior
Photomicrographs of Pseudokeronopsis flava from impregnated specimens. A Ventral view of a typical individual; B Dorsalkineties (arrowheads); C Two frontoterminal cirri (arrowheads); D Buccal cirrus (arrowhead) and undulating membranes; E Infracil-iatureof bicorona (arrows); F Three transverse cirri (arrowhead). Scale bar: A=50 μm.
ends of marginal rows distinctly separated (
Figs.4
F,
6
A). Dorsal bristles length 5-10 μm (
Fig.5
D), three arranged kine-ties, dorsal kineties extending to entire dorsal surface (
Figs.4
G,
6
B).
Cortical granules:
Two types of cortical granules present on both sides (
Figs.4
D,
5
H); yellowish cortical granules form-ing four rows with cirral rows on ventral side (
Figs.4
B,
5
F), small groups with dorsal bristles on dorsal side and 0.8-1 μm in diameter (
Figs.4
C,
5
G, H), colorless cortical granules shaped ellipsoidal and “blood-cell-shaped” under both cortex and about 2×1.5 μm in size (
Fig.5
G, H).
Nuclear apparatus:
70-100 ovoid to ellipsoidal macronuclear nodules, size 5-10 μm long in protargol-impregnated speci-mens. Micronucleus inconspicuous, about 3 μm in diameter(
Figs.4
G,
6
A).
Distribution.
Europe (England, France, Denmark, Germany, Italy, and Poland), North America (USA), and Asia (China and Korea [present study]).
Remarks
. The Korean population of
Pseudokeronopsis flava
closely matched the original and subsequent redescriptions. However, the Korean population differs from the other populations in the pattern of undulating membranes. The undu-lating membranes of the Korean population usually have one intersecting point at the mid part, whereas those of the Chinese population have no intersecting point at the mid part. The length of the endoral membrane is usually longer than that of the paroral in the Korean population, whereas that of both membranes is equal in the Chinese population. However, the lengths of the endoral and paroral membranes of the Danish population are similar to those of the Korean popula-tion (Cohn, 1866; Song et al., 2004, 2006; Sun and Song, 2005; Berger, 2006). The size of the pigment granules is slightly different in the Korean and Chinese populations (0.8-1 μm vs. 0.5 μm) (Song et al., 2004, 2006).
Closely-related species of
Pseudokeronopsis flava
are
P.carnea, P. flavicans,
and
P. rubra
, but they can be distin-guished from each other as follows.
Pseudokeronopsis flava
is different from
P. carnea
in terms of pigment granule color (yellow vs. red), number of bicorona (5-7 vs. 9-11), number of midventral cirri (ca. 25 vs. ca. 40), number of transverse cirri (2-4 vs. 8-9), number of dorsal kineties (3 vs. 7-8), and number of adoral membranelles (ca. 50 vs. ca. 70).
Pseudokeronopsis flava
is different from
P. flavicans
in posterior body shape (slightly narrow vs. distinctly narrow), position of the contractile vacuole (posterior half vs. anterior half),number of dorsal kineties (3 vs. 5), and the gap between the posterior of the midventral row and transverse cirri (wide vs.narrow).
Pseudokeronopsis flava
is different from
P. rubra
in pigment granule color (yellow vs. red) and number of dorsal kineties (3 vs. usually 4-6) (Song et al., 2002, 2004, 2006, Berger, 2006).
Korean name: 1*쌍열충속, 2*붉은쌍열충
Korean name: 1*노랑위각모충
Acknowledgements
This study was supported by the grant from the National Research Foundation of Korea Grant funded by the Korea Government (No. 2009-0077263), and “The Survey of Korean Indigenous Species” supported by the National Institute of Biological Resources (NIBR) of Korea.
Berger H
2001
Catalogue of ciliate names 1. Hypotrichs.
VerlagHelmut Berger
Salzburg
1 -
206
Berger H
2006
Monograph of the Urostyloidea (CiliophoraHypotricha).
Springer Publishers
Dordrecht
1 -
1303
Borror AC
,
Wicklow BJ
1983
The suborder Urostylina Janko-wski (Ciliophora Hypotrichida): morphology systematics and identification of species.
Acta Protozoologica
22
97 -
126
Bütschli O
1889
Protozoa. III. Abtheilung: Infusoria und System der Radiolaria. In: Klassen und Ordnungen des Thier-Reichs wissenschaftlich dargestellt in Wort und Bild Vol.1 (Ed. Bronn HG).
C.F. Winter’sche Verlagsjandlung
Leip-zig and Heidelberg
1585 -
2035
Cohn F
1866
Neue Infusorien im Seeaquarium.
Zeitschrift für Wissenschaftliche Zoologie
16
253 -
302
Corliss JO
1979
The ciliated protozoa: characterization classi-fication and guide to the literature.
Pergamon Press
Londonand New York
1 -
455
Foissner W
1987
Miscellanea nomenclatorica ciliatea (Protozoa;Ciliophora).
Archiv für Protistenkunde
133
217 -
235
Foissner W
1992
Protargol methods. In: Protocols in protozoo-logy(Eds. Lee JJ Soldo AT). The Society of Protozoologists
Allen Press
Lawrence KS
C 6.1 -
C 6.8
Jankowski AW
1979
Revision of the order Hypotrichida Stein1859. Generic catalogue phylogeny taxonomy.
Trudy Zoo-logicheskogo Instituta Akademii Nauk SSSR
86
46 -
85
Jerka-Dziadosz M
,
Janus I
1972
Localization of primordiaduring cortical development in Keronopsis rubra (Ehrbg.1838) (Hypotrichida).
Acta Protozoologica
10
249 -
262
Kahl A
1932
Urtiere oder Protozoa. I: Wimpertiere oder Ciliata (Infusoria) 3. Spirotricha.
Die Tierwelt Deutschlands
25
399 -
650
Kent WS
1882
A manual of the infusoria: including a descrip-tion of all known flagellate ciliate and tentaculiferous pro-tozoa British and foreign and an account of the organization and affinities of the sponges. Vol. 2.
David Bogue
London
721 -
913
Lynn DH
2008
The ciliated protozoa: characterization classi-fication and guide to the literature.
3rd ed.
Springer Publi-shers
New York
1 -
605
Lynn DH
,
Small EB
2002
Phylum Ciliophora. In: An illustrated guide to the Protozoa: organisms traditionally referred to as protozoa or newly discovered groups Vol. 1 (Eds. Lee JJKLeedale GF Bradbury PC).
2nd ed.
Society of Protozoolo-gists
Lawrence
371 -
656
Oberschmidleitner R
,
Aescht E
1996
Taxonomische Untersu-chungenüber einige Ciliaten (Ciliophora Protozoa) aus Belebtschlämmen oberösterreichischer Kläranlagen.
Beiträge zur Naturkunde Oberösterreichs
4
3 -
30
Shao C
,
Song W
,
Li L
,
Warren A
,
Hu X
2007
Morphological and morphogenetic redescriptions of the stichotrich ciliate Diaxonella trimarginata Jankowski 1979 (Ciliophora Sticho-trichia Urostylida).
Acta Protozoologica
46
25 -
39
Song W
,
Sun P
,
Ji D
2004
Redefinition of the yellow hypotri-chous ciliate Pseudokeronopsis flava (Hypotrichida: Cilio-phora).
Journal of the Marine Biological Association of theUnited Kingdom
84
1137 -
1142
DOI : 10.1017/S0025315404010574h
Song W
,
Warren A
,
Roberts D
,
Wilbert N
,
Li L
,
Sun P
,
Hu X
,
Ma H
2006
Comparison and redefinition of four marinecoloured Pseudokeronopsis spp. (Ciliophora: Hypotrichida)with emphasis on their living morphology.
Acta Protozoo-logica
45
271 -
287
Song W
,
Wilbert N
,
Warren A
2002
New contribution to themorphology and taxonomy of four marine hypotrichous ciliates from Qingdao China (Protozoa: Ciliophora).
Acta Protozoologica
41
145 -
162
Sun P
,
Song WB
2005
Morphogenesis of the marine ciliate Pseudokeronopsis flava (Cohn 1866) Wirnsberger et al. 1987 (Protozoa: Ciliophora: Hypotrichida).
Acta ZoologicaSinica
51
81 -
88
Wilbert N
1975
Eine Verbesserte Technik der Protargolimpra-gnation für Ciliaten.
64
171 -
179
Wirnsberger E
,
Larsen HF
,
Uhlig G
1987
Rediagnoses of closely related pigmented marine species of the genus Pseudokero-nopsis (Ciliophora Hypotrichida).
European Journal of Pro-tistology
23
76 -
88
DOI : 10.1016/S0932-4739(87)80010-X