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Role of Glyco-Persica® in Targeting Diabetes Type 2: an Integrative Approach
Role of Glyco-Persica® in Targeting Diabetes Type 2: an Integrative Approach
Journal of Pharmacopuncture. 2013. Oct, 16(4): 14-21
Copyright ©2013, KOREAN PHARMACOPUNCTURE INSTITUTE
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • Received : August 28, 2013
  • Accepted : November 03, 2013
  • Published : October 30, 2013
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About the Authors
Mehrab Dashtdar
Department of Integrative Medicine, Dubai Pharmacy College, Dubai, United Arab Emirates
Mohammad Reza Dashtdar
Emergency Department, International Modern Hospital, Intensive Care Unit, Dubai, United Arab Emirates
Babak Dashtdar
Department of General Medicine, Shiraz University of Medical Science, Shiraz, Iran
Saeed Ahmad Khan
Dubai Pharmacy College, Dubai, United Arab Emirates

Abstract
Objectives:
The objective of this study was to examine how an integrated approach to type 2 diabetes mellitus treatment could improve glycemic control and immune-potentiating activities adherent to oral hypoglycemic agents along with a botanical compound, among primary care patients.
Methods:
In this study, we used the self-control and the group-control methods. Candidates meeting the trial conditions were selected from among volunteers who had taken the test substance for 45 days. During the trial, all groups were on a controlled diet; neither were the original medications nor their dosages changed.
Results:
The results showed that the botanical compound (Glyco-Persica ® ) significantly reduced the main clinical symptoms in diabetes type 2. In the treatment group, 36 of 52 patients (69.23%) and in the control group 10 of 52 patients (19.23%) showed reduced symptoms, and this difference was statistically significant ( P < 0.05). The fasting blood sugar in the treatment group after treatment compared with that before treatment and with that in the control group after treatment was statistically different ( P < 0.05). The post-prandial glucose in the treatment group after treatment was significantly different from that before treatment and from that in the control group after treatment ( P < 0.05); the post-prandial blood sugar in the treatment group was reduced by 8.98%.
Conclusions:
The results revealed that the botanical compound (Glyco-Persica ® ) has significant hypoglycemic properties which affect main clinical symptoms in diabetes type 2. Body weight, blood pressure, heart rate, routine blood, stool and urine tests showed no meaningful negative changes after the course of treatment. There was no significant adverse reaction during the trial.
Keywords
1. Introduction
Diabetes mellitus (DM) is a syndrome of chronic hyperglycemia due to a relative insulin deficiency, insulin resistance, or both. Diabetes is rampant in many parts of the world, with incidence rates increasing yearly. Many chemical agents are available to control and treat diabetes but overall protecting on against long-term complications requires much efforts and money. In addition, the side effects from using chemical hypoglycemic agents, to maintain euglycemia and avoid late-stage diabetic complications disgust most patients. Alternatives to these synthetic agents, plants, provide a potential source of hypoglycemic drugs and are widely used in several traditional systems of medicine to prevent diabetes. The actions of herbal medicines and nutraceuticals are commonly multi-target, multi-channel and synergistic, due to the variety of constituents within a single natural product. Owing to these properties, herbal medicines and nutraceuticals may be beneficial in dealing with diabetes itself, as well as with its complications, because various mechanisms are involved in diabetic vascular complications. [ 1 ]. However, because diabetes is a dangerous disease with many potential complications, alternative treatments for diabetes should not be attempted as a substitute for conventional medical care.
The objectives of this study were to examine how an integrated approach to the treatment of type 2 diabetes mellitus could improve glycemic control, how immune-potentiating activities respond to oral hypoglycemic agents, and whether or not botanical compounds have an adjuvant effect among primary care patients.
2. Materials and Methods
Aqueous extracts of the experimental materials were obtained and mixed in a proper formula. (Table 1 ). Aqueous extract of all ingredients were purchased from Shanghai Wellong Medical Material CO., LTD. (Shanghai, China), and were identified by the Department of Authentication of Dubai Pharmacy College, Alternative Department.
Astragalus membranaceus is an herb that has historically been used in traditional Chinese medicine, usually in combination with other herbs, to support and enhance the immune system. Some other studies found that Astragalus hypoglycemic activities in type 2 diabetic (T2DM) rats. Hypoglycemic effect of APS was investigatedregarding possible mechanism(s) underlying improvement of insulin resistance in vivo and in vitro . [ 2 ] In vivo studies on diabetic rats suggest that increasing peripheral glucose metabolism via Astragalus can alleviate type II diabetes induced by diet with oral doses of 400-700 mg/kg body weight in diabetic rats [ 3 ]. These effects are due to Astragalus Polysaccharides rather than the steroidal saponin content. [ 4 ]
Rehmanniae Radix and Panax ginseng are plant has been used traditionally in Chinese medicine to treat diabetes, with similar claim to that of Astragalus [ 5 - 6 ]. Their combination is being looked at for wound healing in diabetics and the combination of the two is believed to be synergistically beneficial.
The vegetable Momordica charantia L . is also known as bitter gourd, balsam pear, bitter melon. Bitter gourd extracts possesses antioxidant, antibacterial, antiviral, anti- hepatotoxic and anti-ulcerogenic properties as well as the ability to lower blood sugar. Animal and in vitro data support both insulin secretagogue and insulin-mimetic activity of the fruit. A polypeptide (p-insulin) produces hypoglycemic effects in humans and animals by subcutaneous injection, but oral activity are questionable. [ 7 - 10 ] Preliminary animal and human trials suggest possible hypoglycemic and anti-hyperlipidemic properties of oral fenugreek seed powder. In animal and several human trials, fenugreek seeds have been found to lower fasting serum glucose levels, both acutely and chronically. The hypoglycemic effects of fenugreek have been attributed to several mechanisms. [ 11 - 15 ]
Ingredients of the botanical compound used in this study
Latin NamePart extracted% for each 500 mg capsule
Astragalus membranaceus Root 25 %
Salvia miltiorrhiza Root 15 %
Panax Ginseng Root 5 %
Momordica charantia (bitter gourd) unripe fruit and seeds 15 %
Trigonellafoenum-graecum (Fenugreek) Seeds 15 %
Rehmanniae Radix (Sheng Di Huang ) Root 10 %
CoptischinensisFranch (Huang Lian) Root 10 %
Apiscerana Bee wax 5 %
Chromium (III) picolinate 50 microgramsadded to each capsule
The major constituents of Danshen include water-soluble phenolic acids and lipophilic Danshinones. Phenolic acids possesses antioxidant and anticoagulant activities, whereas Danshinones show antibacterial, antioxidant, and antineoplastic activities. [ 16 ] Danshinone enhanced the activity of insulin in Chinese hamster ovary cells and in adipocytes. Pathways through which Dihydrotanshinone- I might affect blood glucose were examined in cellular experiments. Inhibition of advanced glycation end products through alpha-glucosidase blockade by danshen was reported. [ 17 - 19 ]
Some studies show that huanglian has cardiovascular effect. Intravenous injection of berberine at 0.1 to 6.0 mg/kg, lowered blood pressure in anesthetized dogs, cats and rats. The mechanism of blood pressure reduction is dilation of the blood vessels and inhibition of secretion from adrenal glands. Berberine also has antiarrhythmic actions. [ 20 - 21 ] The flavonoids and antioxidant phenols concentrated in Propolis are powerful antioxidants and have been shown to be capable of scavenging free radicals which can extensively interfere with normal cell metabolism. Active free radicals, together with other factors, are considered to be responsible for cellular ageing and degradation in conditions such as cardiovascular diseases, arthritis, cancer, diabetes, and neurodegenerative diseases [ 22 - 25 ].
Chromium (Cr) is an essential element required for normal carbohydrate metabolism. A preliminary study found that treatment with corticosteroids caused increased loss of chromium in the urine [ 26 ]. Another preliminary study found that individuals with corticosteroid-induced diabetes could improve blood sugar control by taking chromium supplements [ 27 ]. Trivalent Cr, the form of Cr found in foods and nutrient supplements, is considered one of the least toxic nutrients. [ 28 ]
Sample no. 1 was a 500-mg capsule of Glyco-Persica. Its ingredients are shown in (Table 1 ).
Sample no. 2 was a 500-mg placebo capsule, filled with post-extracted washed fibers. Two capsules (1000 mg) were administered three times daily for 45 days.
The inclusion criteria were age 24-70, fasting blood sugar > 200 mg /dl, 2 h postprandial blood sugar > 200 mg/dl, history of diabetes type 2 of more than one year and treated with allopathic medications.
Exclusion criteria were pregnancy, severe heart failure, renal failure, liver failure and other complications. Patients with other primary diseases, such as diabetic ketoacidosis and who under taken corticosteroids or other medicines that affect blood sugar blood sugar were also excluded.
We used double blind random selection to divide the patients who had been treated between March 2007 and November 2009 and who were willing to participate in this trial; according to their blood glucose into treatment and a control groups. A balanced test among all factors that might affect the course of disease such as medication, Type, gender and age, was done to avoid comparability between each two groups. All participants took medicine every day for 45 days. All indices were measured before and after intake of food. Safety indicators included general physical examinations:
Detailed inquiries regarding participant’s mental condition, sleep routine, diet, urine and stool, body weight, blood pressure, and heart rhythm before the start of this study; complete blood count assessments: red blood cells, white blood cells and differentiation, and hemoglobin.
Routine stool tests were done and included routine urine analysis: pH, white blood cell count (WBC), sugar, and ketones.
Blood biochemical tests for serum total protein, albumin, alanine transaminase, aspartate transaminase, blood urea nitrogen, serum creatinine, blood glucose, total cholesterol, triglycerides, and high-density lipoproteins.
Chest x-rays, electrocardiograms, abdominal sonograms were taken before the test. Finally, the side effects of medications were observed.
Detailed inquiries were made about the participant’s history, diet, medications, and activities. In all cases, we determined the chief complaint such as polydipsia, polyuria, polyphagia, fatigue, etc., before and after food, and we divided the symptoms into three categories according to severe as 3, medium as 2 and mild as 1. We also observed symptom improvement. (Improvement of more than one for each symptom was considered effective.)
Glucose tolerance tests were done before and 2 h after the consumption of 10 large dates or bread made from 100 gram of flour.
Urine sugar and ketones were measured. Morning urine was measured on an empty stomach, and according to positive rate, the results were divided into - , ±, +, ++, +++, ++++ groups corresponding to 0, 0.5, 1, 2, 3, and 4 points, respectively. Before and after food, we statistically analyzed the integral values.
Basic symptoms were considered to be reduced significantly in the treatment group when a significant difference in the fasting blood sugar or the 2-h post-prandial blood glucose existed between the treatment and the control groups or between the treatment group before and after treatment. A drop of ≥10 % compared to the pre-test blood sugar was considered a significant difference.
Results are expressed as means ± standard errors. Paired sample t -tests, under the premise of homogeneity of variance, were compared using composition t -tests; otherwise, variables were transformed to satisfy homogeneity of variance, after which t -tests were used. If the variance was still missing, a Wilcoxon signed-rank test was used. Pearson’s chi-squared test (χ2) was used for efficiency.
3. Result
Double-blind observations were made. Capsule 1 was used for the treatment group, and capsule 2 for the placebo group. In general, the two groups were comparable (Table 2 )
Clinical symptom scoring and clinical symptoms with changes are shown in Table 3 and 4 , respectively. Clinical symptoms were compared between the treatment group before and after treatment and between the treatment and the control groups after treatment, and the differences were statistically significant ( P < 0.05). In the treatment and the control groups, the symptoms were improved in 36 (69.23%) and 10 (19.23%) of 52 cases, respectively, a significant difference ( P < 0.05) (Tables 3 , 4 )
Before treatment, the fasting blood sugar levels in the two groups were not significantly different ( P > 0.05); neither were the fasting blood sugar levels in the control group before and after treatment ( P > 0.05). However, the fasting blood glucose level in the treatment group after treatment compared with that before treatment and with that of the control group after treatment was significantly different ( P < 0.05). After treatment, the blood glucose levels in the treatment and control groups were lower by 21.6 mg/dl (12.67% reduction) and 1.98 mg/dl (1.16% reduction), respectively, the difference being statistically significant ( P <0.05) (Table 5 ).
General characteristics and conditions before treatment
Characteristic/conditionControl group% for each 500 mg capsule
The number of cases 53 53
Male/Female 28/25 27/26
Age (yr) 53.90 ± 9.64 54.51 ± 8.69
Duration of disease (yr) 4.79 ± 1.79 4.77 ± 1.80
Not using medicine 2 1
Sulfonylurea 15 14
Biguanide (Metformin) 16 15
Sulfonylurea+ Biguanide 19 21
Others 1 2
Clinical symptom scoring (integral value, mean ± SD)
GroupsBefore treatmentAfter treatment
Control Group 6.77 ± 2.67 6.58 ± 2.51
Treatment Group 6.79 ± 2.64 5.67 ± 1.94*
* This value was statistically different from the value before treatment in the treatment group and from the value after treatment in the control group (P < 0.05).
Changes in clinical symptoms
SymptomsControl groupTreatment groupImprovement rate (%)
CasesEffectiveNot effectiveCasesEffectiveNot effectiveControl groupTreatment group
Polyphagia 46 2 44 44 13 31 4.35 29.55
Polydipsia 48 2 46 49 14 33 4.17 29.79
Fatigue 47 3 44 48 15 33 6.38 31.25
Polyuria 49 3 46 49 16 33 6.12 32.65
Total  52  10  42  52  36  16  13.23  69.23
* Compared with control group, the difference was statistically significant (P < 0.05).
Before treatment, the post-prandial blood glucose levels in the two groups were not significantly different ( P > 0.05). In the control group, the levels before and after treatment were not significantly different ( P > 0.05). However, in the treatment group, the level after treatment compared with that before treatment and with that of the control group after treatment was significantly different ( P < 0.05). In the treatment and control groups, the levels were reduced by 20.88 mg/dl and 1.62 mg/dl, respectively, the difference being statistically significant ( P < 0.05) (Table 6 ).
In both the two groups, the urine glucose levels after treatment were not significantly different. Also, in the treatment and the control groups, the levels before and after treatment were not significantly different ( P > 0.05). These results indicate that this botanical compound had no significant effect on glycosuria. Before and after treatment, Ketonuria was not detected (Table 7 ).
Body weight, blood pressure, heart rate, routine blood and stool/urine checkups (except urine glucose), and biochemical tests showed no apparent changes. No significant adverse reactions occurred during evaluations. The Glyco-Persica medication caused no obvious damage to the body (Table 8 ).
After 45 days of treatment, one case each in the groups was eliminated due to intermittent use the placebo and the herbal compound such that we could not determine a result. The final number of patients in each group was 52 (Table 9 ).
Fasting blood sugar levels before and after treatment (mg/dl, mean ± SD)
GroupBefore treatment mg/dlAfter treatment mg/dlDecrease in blood glucosePercent decrease inblood glucose
Control group 170.28 ± 49.5 168.12 ± 48.24 1.98 ± 4.68 1.16
Treatment Group 170.46 ± 51.66 148.86 ± 48.78* 21.6 ± 5.04** 12.67
*This value is statistically different from the value in the treatment group before treatment and from the value in the control group after treatment (P < 0.05). **This value is statistically different from the corresponding value for the control group (P < 0.05).
Post-prandial blood glucose changes (mg/dl, Mean ± SD)
GroupBefore treatmentmg/dlAfter treatmentmg/dlDecrease in bloodglucosePercentage reduction(%)decrease in bloodglucose
Control group 234.36 ± 50.76 232.56 ± 51.66 1.62 ± 6.3 0.69
Treatment Group 232.56 ± 52.2 211.86 ± 49.86* 20.88 ± 5.04** 8.98
*This value is statistically different from the value in the treatment group before treatment and from the value in the control group after treatment (P < 0.05). **This value is statistically different from the corresponding value for the control group (P < 0.05). Compared with before treatment and with control group, the differences were statistically significant (P < 0.05).
Evaluation of glycosuria and urine ketone before and after treatment (mean ± SD)
MeasurementGroupBefore treatmentAfter treatment
Glycosuria Control group 1.38 ± 0.66 1.35 ± 0.65
Treatment group 1.40 ± 0.63 1.33 ± 0.62
Urine ketone Control group Negative Negative
Treatment group Negative Negative
4. Discussion
The results showed that Glyco-Persica significantly lessened the main clinical symptoms in diabetes type 2 patients (treatment group: 69.23%, control group: 19.23%). The fasting blood sugar in the treatment group after treatment was significantly different from that in the treatment group before treatment and from that in the control group after treatment ( P < 0.05). In the treatment group, the reduction in the fasting sugar after treatment was significant compared to that in the control group (12.67% vs. 1.16%, P < 0.05). Also, the post-prandial sugar in the treatment group after treatment compared with that before treatment and with that in the control group after treatment was significantly different (8.98% vs. 0.69%, P < 0.05).
5. Conclusion
The results revealed that Glyco-Persica ® had a significant hypoglycemic effect on the main clinical symptoms in diabetes type 2 patients, but body weight, blood pressure, heart rate, routine blood and stool/urine checkups, and biochemical tests showed no apparent changes. No significant adverse reactions were noted during evaluations.
Analysis of body weight, blood pressure, heart rate, routine blood, stool/urine and blood biochemical index before and after treatment (mean ± SD)
Measurement Control groupTreatment group
Before treatmentAfter treatmentBefore treatmentAfter treatment
Body weight (kg)  64.14 ± 12.81 64.09 ± 12.75 62.09 ± 9.50 62.06 ± 9.52
Blood pressure (mmHg) Maxi 110.40 ± 7.20 110.69 ± 7.98 110.69 ± 7.56 110.44 ± 8.09
Mini 75.56 ± 6.96 75.92 ± 7.36 76.60 ± 6.82 76.38 ± 7.41
Heart rate (times/ min)  75.83 ± 6.31 75.44 ± 6.46 74.06 ± 7.76 73.58 ± 8.45
White blood cells ( x109/L)  6.33 ± 1.52 6.48 ± 1.26 6.10 ± 1.32 6.18 ± 1.20
Red blood cells ( x109/L)  4.36 ± 0.36 4.39 ± 0.35 4.43 ± 0.35 4.47 ± 0.36
Hemoglobin (mg/dL)  132.65 ± 10.28 132.79 ± 10.16 134.96 ± 10.97 135.10 ± 10.92
Urine routine (except glucose)  Normal Normal Normal Normal
Stool routine  Normal Normal Normal Normal
Total protein serum (g/L)  73.34 ± 2.88 73.29 ± 2.88 73.58 ± 2.61 73.66 ± 2.66
Serum albumin (g/L)  42.97 ± 2.73 42.91 ± 2.56 43.47 ± 2.69 43.48 ± 2.58
Alanine transaminase (IU/L)  31.94 ± 14.96 30.25 ± 12.90 27.71 ± 15.56 25.67 ± 12.00
Aspartate transaminase (IU/L)  30.25 ± 12.23 28.69 ± 10.42 26.90 ± 12.93 26.79 ± 11.31
Blood urea nitrogen (mmol/L)  5.37 ± 1.05 5.62 ± 0.93 5.53 ± 1.00 5.61 ± 1.05
Serum creatinine (μmol/L)  83.81 ± 13.37 84.04 ± 11.54 83.04 ± 13.02 84.21 ± 11.82
Uric Acid (μmol/L)  251.67 ± 58.88 253.10 ± 55.59 233.12 ± 54.44 243.29 ± 54.04
Cholesterol (mmol/L)  5.37 ± 0.98 5.39 ± 0.96 5.16 ± 0.90 5.14 ± 0.81
Triglycerides (mmol/L)  1.96 ± 0.69 1.94 ± 0.71 1.82 ± 0.71 1.80 ± 0.71
Trail screening rate
GroupControl groupTreatment group
Before treatment 53 53
Screened out 1 1
screening rate 1.89% 1.89%
Acknowledgements
We are grateful to Havva Dashtdar, PhD for her improving the paper. We express our sincere thanks to Nader and Esmail Dashtdar for providing the herbal extract. Finally, we thank Somayeh Rasooli and Samira Fathizadeh for their encouragement and insights. No competing financial interests exist.
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