Effect of Porcine Collagen Peptides on the Rheological and Sensory Properties of Ice Cream
Effect of Porcine Collagen Peptides on the Rheological and Sensory Properties of Ice Cream
Food Science of Animal Resources. 2015. Apr, 35(2): 156-163
Copyright © 2015, Korean Society for Food Science of Animal Resources
  • Received : October 08, 2014
  • Accepted : December 04, 2014
  • Published : April 30, 2015
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Liying, Li
Jae-Hyeong, Kim
Yeon-Ji, Jo
Sang-Gi, Min
Ji-Yeon, Chun

The effects of low molecular-weight collagen peptides derived from porcine skin were investigated on the physicochemical and sensorial properties of chocolate ice cream. Collagen peptides less than 1 kDa in weight were obtained by sub-critical water hydrolysis at a temperature of 300℃ and a pressure of 80 bar. Ice cream was then prepared with gelatin powder and porcine skin hydrolysate (PSH) stabilizers mixed at seven different ratios (for a total of 0.5 wt%). There was no significant difference in color between the resulting ice cream mixtures. The increase in apparent viscosity and shear thinning of the ice cream was more moderate with PSH added than with gelatin. Moreover, the samples containing more than 0.2 wt% PSH had enhanced melting resistance, while the mixture with 0.2 wt% PSH had the lowest storage modulus at -20℃ and the second highest loss modulus at 10℃, indicating that this combination of hydrocolloids leads to relatively softer and creamier chocolate ice cream. Among the seven types of ice creams tested, the mixture with 0.2 wt% PSH and 0.3 wt% gelatin had the best physicochemical properties. However, in sensory evaluations, the samples containing PSH had lower chocolate flavor scores and higher off-flavor scores than the sample prepared with just 0.5 wt% gelatin due to the strong off-flavor of PSH.
Ice cream is a frozen product consisting of air cells, ice crystals, and fat droplets dispersed in a serum phase. During processing, ice cream is frozen rapidly by removing heat from the mix while stirring to incorporate air ( Marshall ., 2003 ). Stabilizers, predominantly hydrocolloids, are used during preparation to provide a smooth texture, for shape retention during melting, and to increase the viscosity of the mix ( Goff and Hartel, 2004 ; Marshall ., 2003 ). Most importantly, these cryoprotective hydrocolloids retard the growth of ice crystals during storage, especially during temperature fluctuations, but they neither change the thermodynamics of the ice cream mix nor alter ice nucleation kinetics ( Marshall ., 2003 ; Muhr ., 1986 ).
Gelatin, derived from collagen obtained from various animal by-products, is one of the principal materials used as an ice cream stabilizer. It disperses easily and does not cause wheying-off or foaming. However, gelatin is a relatively expensive stabilizer only effective at high concentrations and tends to offer poor protection against the effects of heat shock. It also requires prolonged aging ( Wang and Damodaran, 2009 ). With these drawbacks in mind, low molecular-weight (600-2,700 Da) collagen peptides derived from Alcalase hydrolysis of bovine gelatin have been found to inhibit ice recrystallization in a supercooled ice cream mix ( Wang and Damodaran, 2009 ). These can therefore be considered as new stabilizers for ice cream production.
Other than their use as stabilizers, collagen peptides have also attracted attention for their bioactive properties. An antihypertensive peptide has been obtained from porcine skin collagen ( Ichimura ., 2009 ). Numerous studies have investigated the antioxidant properties of peptides obtained from collagenous sources; these may protect living cells against free radical mediated oxidative damage ( Kim ., 2001 ).
Subcritical water, viz. pressurized water heated above its boiling point but below the critical point of 374℃ and 221 MPa, has unique properties ( Lee ., 2013 ). Com-pared with other hydrolysis methods, the “green” approach of using water as a solvent in place of hazardous substances also expedites processing ( Alargov ., 2002 ; Lee ., 2013 ). Sub-critical water hydrolysis has been employed to efficiently extract amino acids from collagen ( Zhu ., 2011 ). Furthermore, the effects of high pressure/high temperature (sub-critical water) treatment on porcine placenta have also been reported ( Lee ., 2013 ).
In this study therefore, low molecular-weight collagen derived from porcine skin (< 1kDa, porcine skin hydrolysate, PSH) was extracted using sub-critical water (300℃ and 80 bar). Chocolate ice cream was then prepared using PSH which contained 5 wt% collagen peptides as a stabilizer and cocoa powder because of its strong off-flavor. The effects of different PSH contents on the physicochemical and sensorial properties of chocolate ice cream were analyzed.
Materials and Methods
- Preparation of porcine skin hydrolysates
Porcine skin hydrolysates (PSH) were produced by subcritical water hydrolysis. The treatment conditions were optimized in a previous study ( Kim , 2014 ). Porcine skin was bought from a local butcher’s shop (Daeho Chooksan, Korea). The skin was immersed in 70℃ water for 2 h to remove fat and other residues and then sliced into 0.5 cm × 0.5 cm pieces. These were homogenized first for 3 min using a four-wing blade blender (CNHR- 26, Bosch, China) and then at 25,000 rpm for 5 min using an Ultra-Turrax® blender (T25, IKA Labotechnik, Germany). The sub-critical water system (300°C and 80 bar) was composed of a control box, vessel (reactor), water bath, heater, temperature controller, and pressure controller (SFE SYSTEM, CS-1000, REXO, Korea). Porcine skin mixed with distilled water (1:2, w/w) was then hydrolyzed and cooled directly in this system whose temperature and pressure were reduced down to 40-45℃ and 0.1 bar, respectively in a 4℃ water bath with coolant circulation. All processing steps were completed within 1 h.
- Characterization of the porcine skin hydrolysates
All characterizations were performed as described by Kim . (2014) . The samples were centrifuged at 10,000 g for 5 min and the molecular weights of the peptides in the supernatant were determined by gel permeation chromatography (GPC, YL 9100, Younglin Instrument Co. Ltd., Korea). The amino acid profile was determined at the Animal Resources Research Center in Konkuk University (S4300 Amino Acid Reaction Module, Systeme & Komponenten analytischer Meßtechnik, Germany). The free amino group content was determined using the method of Benjakul and Morrissey (1997) , and expressed in terms of L-leucine ( Nagarajan ., 2012 ). The pH and color of the filtered PSH solution were respectively determined using a pH meter (Model S220, Mettler Toledo GmbH, Switzerland) and a colorimeter (CR-210 Chroma-meter, Minolta, Japan) calibrated using a white standard (CIE L * = +97.83, CIE a *= −0.43, CIE b *= +1.96).
- Ice cream preparation
Different ice cream samples were prepared with the stabilizer type and content varied as follows: 0.5 wt% gelatin (Hayashi Pure Chemical Industries Ltd., Japan), 0.5 wt% PSH, PSH 0.1 (0.1 wt% PSH+0.4 wt% gelatin), PSH 0.2 (0.2 wt% PSH+0.3 wt% gelatin), PSH 0.25 (0.25 wt% PSH+0.25 wt% gelatin), PSH 0.3 (0.3 wt% PSH+ 0.2 wt% gelatin), and PSH 0.4 (0.4 wt% PSH+0.1 wt% gelatin). The ice cream used in this study consisted of 11 wt% milk fat and 59.3 wt% water (provided as milk and whipped cream, Maeil Dairies Industry Co., Ltd., Korea), 10 wt% non-fat milk solids (skimmed milk powder, Maeil Dairies Industry Co., Ltd., Korea), 12 wt% sucrose (CJ CheilJedang Corp., Korea), 4 wt% corn syrup solids (Dextrose Equivalent 42, Grain Processing Corp., USA), 0.2 wt% glyceryl monosearate (Daejung Chemicals & Metals Co., Ltd., Korea), and 3 wt% cocoa powder (ADM Cocoa Pte. Ltd., Singapore) ( Table 1 ).
Composition of the chocolate ice cream samples used in the present study
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Composition of the chocolate ice cream samples used in the present study
All ingredients were mixed, pasteurized at 70℃ for 30 min, and then homogenized using a high-speed homogenizer (Ingenieurbüro CAT M. Zipperer GmbH, Germany) for 30 min at 11,000 rpm. The ice cream mix was then cooled down to 4℃ and aged overnight. The aged ice cream mix was frozen in a freezer (Sani-serv 407 1E, USA) at a drawer temperature of −5℃, and packed into 30 mm × 5 mm containers and 120 mL specimen cups (SPL Lifesciences Co., Ltd., Korea). The ice creams were hardened and stored at −20℃ for 24 h in a freezer (CRFD- 0621, Samsung Electronics Co., Ltd., Korea). The overall experimental procedure was repeated three times giving a total of 21 formulations.
- Rheological properties
- Ice cream viscosity
A rheometer (Anton Paar, MCR 302, Austria) with a concentric cylinder (CC27) was used to determine the viscosity of the ice cream mixes after aging. The measurements were carried out for shear rates ranging from 1 s –1 to 100 s –1 at 4℃. The apparent viscosity (Kokini viscosity) of the ice cream mixes, representing the feeling of low-viscosity foods in the mouth, was calculated at a shear rate of 50 s –1 ( Akhtar ., 2006 ; Kokini 1987 ; Stanley and Taylor, 1993 ). The Ostwald-de Waele model was used to relate the shear rate and the shear stress, through which the consistency coefficient (K) and flow behavior index (n) can be calculated as follows:
σ = Kγ n
where σ is the shear stress (Pa), K is the consistency coefficient (Pa·s n ), γ is the shear rate (s –1 ), and n is the flow behavior index (dimensionless).
- Oscillatory rheometry
Oscillatory thermo-rheometry measurements were carried out ( Wildmoser ., 2004 ) using a rheometer (Anton Paar, MCR 302, Austria) with a plate-plate geometry (PP25-S, plate diameter: 25 mm) to determine the dynamic rheological properties of the ice cream mixtures. A movable hood covering the plate-plate setup reduced temperature fluctuations with the environment. The test was performed at a constant strain γ = 0.2% and angular frequency ω = 10 s –1 . A 2 mm gap was maintained between the plates. The temperature was gradually increased from 20℃ to 10℃ at a constant heating rate of 2℃/min. The storage modulus G' and the loss modulus G" were obtained from measurements, which respectively characterize the elastic and viscous behaviors of the ice cream.
- Melting test
Each time one sample was placed on a stainless mesh on top of a plastic funnel attached to a stand, and a plastic sample dish was placed on an electronic balance (BL- 220H, Shimadzu Corp., Japan). This ice-cream melting system was placed in an incubator (SW-90F, Sangwoo, Korea) maintained at 25℃. The balance was connected to a computer and the weight of the ice cream melt was automatically calculated every minute ( Fig. 1 ).
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Schematic diagram of the ice cream melting system.
- Color differences
The difference in color of the ice cream after hardening was measured using a Chroma-meter (CR-400, Konica Minolta Optics, Inc., Japan). Results were expressed using the L * a * b * scale, for lightness, redness, and yellowness, respectively. The measurements were calibrated to a white standard (CIE L *=+94.50, CIE a *=–0.53, CIE b *= +3.14) and the total color difference (∆ E *) was calculated using the following equation:
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where ( L 1 *, a 1 *, b 1 *) is the reference color and ( L 2 *, a 2 *, b 2 *) is the target color.
- Sensory properties
Sensory characteristics including the appearance, chocolate flavor, off-flavor, bitterness, creaminess, iciness, hardness, sweetness, smoothness, and coarseness of the ice cream were evaluated by trained panelists. In these sessions, the attributes and assessment techniques were defined and a practical sample evaluation was performed. Sensory evaluations were carried out using a 5-point scale, with 1 representing “non-existent or imperceptible” and 5 representing “very intense” properties.
- Statistical analysis
All assays were carried out in triplicate. The data were expressed as means with standard deviations and statistical analysis was performed using the Origin Pro 8 (OriginLab, USA) and SPSS 20.0 (SPSS Institute, USA) software. Means were subjected to analysis of variance by Duncan’s multiple range test and Least-significant difference at p <0.05.
Results and Discussion
- Physical properties of porcine skin hydrolysates
In this study, PSH was produced through an optimized sub-critical water treatment (300℃ and 80 bar) and the physical properties of the resulting PSH were similar to those described in our previous report ( Kim ., 2014 ). Representative characteristics of the PSH used here to prepare ice cream are shown in Table 2 and Fig. 2 . The relatively high pH, 9.22, is due to the increased self-ionization of water during sub-critical water treatments ( Brunner, 2009 , 2014 ; Penninger ., 2000 ; Ravber ., 2015 ; Watchararuji ., 2008 ). The molecular weight peaks obtained following the sub-critical water treatment of PSH are distributed between 434 Da and 626 Da. The free amino group content was 57.18 mM, with the concentrations of Gly (28%) and Ala (13.1%) being the highest. In terms of color, the lightness, redness and yellowness of the PSH were 33.6, 0.59, and 11.4, respectively.
Physical properties of porcine skin collagen extract
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Physical properties of porcine skin collagen extract
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Gel permeation chromatography graph obtained for PSH with crosses indicating the molecular weights of constituents.
- Viscosity of the ice cream mixes
Viscosity, one of the most important rheological properties of ice cream mix, is influenced by many factors notably its composition and the stabilizer type and content. By forming a three-dimensional network of hydrated molecules, hydrocolloids enhance the water-binding capacity of the ice cream mix ( Kus ., 2005 ). Table 3 shows the values measured for the Kokini viscosity, consistency coefficient, and flow behavior index of the ice cream mixes prepared with different PSH-to-gelatin ratios. The flow behavior indexes are all less than one, characteristic of the shear thinning behavior reported previously ( Kaya and Tekin, 2001 ). Ice cream mixes with higher consistency coefficients are considered more viscous. Soukoulis . (2009) related enhanced apparent viscosity by increased Kokini viscosities and consistency coefficients, and increased shear thinning by lower flow behavior indexes. Here, the Kokini viscosity and consistency coefficient increases and the flow behavior index decreases for lower PSH (higher gelatin) concentrations, indicating that PSH leads to a more moderate increase in ice cream viscosity than gelatin. This may be due to the low peptide content of collagen, only 5 wt% in PSH. There is a significant ( p <0.05) difference between the Kokini viscosities of the control, PSH 0.1, and PSH 0.2 ice cream mixes. The control sample has the highest Kokini viscosity (0.43±0.06 Pa·s), followed by PSH 0.1 (0.28±0.07 Pa·s) and PSH 0.2 (0.19±0.03 Pa·s). However, although the other mixes had lower Kokini viscosities, these differences were not significant ( p >0.05).
Effect of hydrocolloids on the Kokini viscosity, consistency coefficient, and flow behavior index of different chocolate ice cream mixesMean±SD values were calculated based on at least 3 repeat measurements.a-dDifferent letters in the same column indicate significantly different values (p<0.05).
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Effect of hydrocolloids on the Kokini viscosity, consistency coefficient, and flow behavior index of different chocolate ice cream mixes Mean±SD values were calculated based on at least 3 repeat measurements. a-dDifferent letters in the same column indicate significantly different values (p<0.05).
- Oscillatory rheometry
Oscillatory tests are used to determine the viscoelastic properties of complex food systems ( Dolz ., 2006 ). Temperature has an important impact on the structure of ice cream, with ice crystal microstructures dominating the rheological behavior of frozen ice cream in the low temperature range, from –20℃ to –10℃. Thus, the storage modulus G' and loss modulus G" below –10℃ report on the ice crystal size and rigidity of the ice cream. As ice is melted away under constant heating from 0℃ to 10℃, the loss modulus G’’, which characterizes the viscosity of the ice cream, can be correlated with creaminess ( Wildmoser ., 2004 ).
The influence of hydrocolloids on the ice cream storage modulus at –20℃ and its loss modulus at 10℃ are presented in Figs. 3 and 4 , respectively. The PSH 0.2 and PSH 0.4 samples have the lowest storage modulus at –20℃ (0.45±0.03 MPa and 0.48±0.03 MPa, respectively). These two combinations of stabilizers are therefore the most effective at inhibiting ice crystal growth during ice cream preparation. The control ice cream mix with the highest Kokini viscosity is also the creamiest, with the highest loss modulus at 10℃ (34.01±2.14 Pa, p <0.05) among the different samples, but also with the largest ice crystals as revealed by the highest storage modulus at –20℃ (1.11± 0.03 MPa, p <0.05). The second and third most viscous mixes (PSH 0.1 and PSH 0.2) are also creamy with no significant difference ( p >0.05) between their loss modulus at 10℃ (21.27±1.25 Pa and 20.63±3.11 Pa, respectively).
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The influence of hydrocolloids on the ice cream storage modulus at –20℃.

Control, 0.5 wt% gelatin; PSH 0.1, 0.1 wt% PSH+0.4 wt% gelatin; PSH 0.2, 0.2 wt% PSH+ 0.3 wt% gelatin; PSH 0.25, 0.25 wt% PSH+0.25 wt% gelatin; PSH 0.3, 0.3 wt% PSH+0.2 wt% gelatin; PSH 0.4, 0.4 wt% PSH+0.1 wt% gelatin; PSH 0.5, 0.5 wt% PSH. Mean±SD values were calculated based on at least 3 repeat measurements. Different letters indicate significantly different (<0.05) values.

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The influence of hydrocolloids on the ice cream loss modulus at 10℃.

Control, 0.5 wt% gelatin; PSH 0.1, 0.1 wt% PSH+0.4 wt% gelatin; PSH 0.2, 0.2 wt% PSH+0.3 wt% gelatin; PSH 0.25, 0.25 wt% PSH+0.25 wt% gelatin; PSH 0.3, 0.3 wt% PSH+0.2 wt% gelatin; PSH 0.4, 0.4 wt% PSH+0.1 wt% gelatin; PSH 0.5: 0.5 wt% PSH. Mean±SD values were calculated based on at least 3 repeat measurements. Different letters indicate significantly different (<0.05) values.

- Melting resistance of the ice cream
Melting resistance is an important parameter used to evaluate the physical stability of frozen ice cream. According to Marshall . (2003) , hydrocolloids improve the resistance to melting due to the enhancement of water holding and microviscosity they provide. However, as shown in Table 4 , although the ice creams with higher viscosities (control and PSH 0.1) have longer first-dropping times (35.67±2.08 min and 23.33±2.52 min, respectively), their melting rates (6.35±0.78 g/min and 4.43± 0.16 g/min, respectively) are significantly higher than those of the other five types of ice cream ( p <0.05). This result is in agreement with Kilara and Chandan (2008) , and proves that gelatin is not effective in preventing the effects of heat shock. The ice cream with the third-highest viscosity (PSH 0.2) has a first dropping time and melting rate that are not significantly different ( p >0.05) from the values measured for the four remaining samples, indicating that melting resistance is similarly enhanced for PSH concentrations above 0.2 wt% and gelatin concentrations below 0.3 wt%.
Effect of hydrocolloids on the melting quality of different chocolate ice cream mixes
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Mean±SD values were calculated based on at least 3 repeat measurements. a-cDifferent letters in the same column indicate significantly different (p<0.05) values.
- Color differences
In this study, since the PSH are dark brown, the lightness, redness, and yellowness of the ice cream mixtures were measured to evaluate the effects of hydrocolloids on the color of hardened chocolate ice cream. Note that color differences in fresh ice cream have seldom been investigated. As Table 5 shows, there is no significant difference in color ( p >0.05) between the ice creams prepared with different PSH to gelatin ratios, with an average lightness, redness, and yellowness of 42.3, 10.5, and 17.1, respectively. Cocoa powder is similar in color to PSH but darker, therefore varying the PSH concentration does not affect the color of the ice cream. In previous tests of the effect of PSH on vanilla ice cream, its color quality was deemed not acceptable because of stains and darkening.
Effect of hydrocolloids on lightness, redness, yellowness and total color difference of chocolate ice cream
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Mean±SD values were obtained from at least 3 repeat measurements. None of the values are significantly different (p>0.05).
- Sensory evaluation
The perceived texture and flavor of ice cream are considered to be the most important factors influencing ice cream consumption. In addition to their other effects, hydrocolloids influence the sensory properties of ice cream ( Donhowe ., 1991 ; Rincon ., 2006 ; Soukoulis ., 2008 ). Comparing the physicochemical properties of the different fresh samples, the PSH 0.2 composition has a relatively high Kokini viscosity, enhanced resistance to melting, the lowest storage modulus at –20℃, and the second highest loss modulus at 10℃. This mixture was therefore chosen for sensory evaluations along with the control and PSH 0.5 samples.
As shown in Table 6 , there was no significant difference ( p >0.05) between the three samples in terms of appearance, bitterness, creaminess, iciness, sweetness, smoothness, or coarseness. Morris (1995) reported that increased shear thinning of hydrocolloids may be associated with better flavor perception. Indeed, in the present study, the sample with 0.5 wt% gelatin, which exhibited the most shear thinning, gained a significantly higher chocolate flavor score ( p <0.05). As expected since PSH is known to cause strong off-flavors, the PSH 0.2 and PSH 0.5 mixtures have significantly higher off-flavor scores ( p <0.05) compared with the control ice cream. The ice cream produced with 0.5 wt% gelatin has a significantly higher hardness score ( p <0.05) than the other two, which is in agreement with the oscillatory rheometry tests that ice crystal growth is not inhibited effectively in the 0.5 wt% gelatin sample.
Effect of hydrocolloids on the sensory attributes of different chocolate ice cream mixtures
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Mean±SD values were calculated based on at least 5 repeat measurements. a,bDifferent letters in the same row indicate significantly different values (p<0.05).
This study analysed the impact of different PSH-to-gelatin ratios on the physicochemical and sensorial properties of chocolate ice cream. In terms of the physicochemical properties, the ice cream prepared with 0.2 wt% PSH and 0.3 wt% gelatin was optimal among the seven compositions tested, with a relatively high Kokini viscosity, enhanced melting resistance, the lowest storage modulus at –20℃, and the second highest loss modulus at 10℃. However, in the sensory evaluations, the control ice cream (0.5 wt% gelatin) was perceived to have the highest chocolate flavor and the lowest off-flavor, possibly due to the strong off-flavor of PSH. The addition of low molecularweight collagen peptides derived from porcine skin was therefore effective in improving the physical and rheological properties of the ice cream, but its sensory properties were degraded by the off-flavor of PSH. Masking the off-flavor of PSH is therefore an important goal for further studies in order to manufacture ideal ice cream products.
Financial support for this study was obtained from the Korean Institute of Planning and Evaluation for Technology in Food, Agriculture, Forest, and Fisheries, Korea(iPET Project No. 311029-3) and this paper was supported by the KU Research Professor Program of Konkuk University
Akhtar M. , Murray B. S. , Dickinson E. 2006 Perception of creaminess of model oil-in-water dairy emulsions: Influence of the shear-thinning nature of a viscosity-controlling hydrocolloid Food Hydrocolloid. 20 839 - 847    DOI : 10.1016/j.foodhyd.2005.08.006
Alargov D. , Deguchi S. , Tsujii K. , Horikoshi K. 2002 Reaction behaviors of glycine under super- and subcritical water conditions Orig. Life Evol. Biosph. 32 1 - 12    DOI : 10.1023/A:1013906319253
Benjakul S. , Morrissey M. T. 1997 Protein hydrolysates from pacific whiting solid wastes J. Agric. Food Chem. 45 3423 - 3430    DOI : 10.1021/jf970294g
Brunner G. 2009 Near critical and supercritical water. Part I. Hydrolytic and hydrothermal processes J. Supercritical Fluid. 47 373 - 381    DOI : 10.1016/j.supflu.2008.09.002
Brunner G. , Brunner G. 2014 Properties of pure water Elsevier Supercritical fluid science and technology 9 - 93
Dolz M. , Hernández M. , Delegido J. 2006 Oscillatory measurements for salad dressings stabilized with modified starch, xanthan gum, and locust bean gum J. Appl. Polym. Sci. 102 897 - 903    DOI : 10.1002/app.24125
Donhowe D. P. , Hartel R. W. , Bradley R. L. 1991 Determination of ice crystal size distributions in frozen desserts J. Dairy Sci. 74 3334 - 3344    DOI : 10.3168/jds.S0022-0302(91)78521-4
Goff H. D. , Hartel R. W. , Hui Y. H. 2004 Handbook of Frozen Foods Marcel Dekker Inc NY Ice cream and frozen desserts 499 - 570
Ichimura T. , Yamanaka A. , Otsuka T. , Yamashita E. , Maruyama S. 2009 Antihypertensive effect of enzymatic hydrolysate of collagen and Gly-Pro in spontaneously hypertensive rats Biosci. Biotechnol. Biochem. 73 2317 - 2319    DOI : 10.1271/bbb.90197
Kaya S. , Tekin A. R. 2001 The effect of salep content on the rheological characteristics of a typical ice cream mix J. Food Eng. 47 59 - 62    DOI : 10.1016/S0260-8774(00)00093-5
Kilara A. , Chandan R. C. , Chandan R.C. 2008 Dairy Processing & Quality Assurance Wiley-Blackwell Oxford Ice cream and frozen desserts 364 - 365
Kim J. H. , Jeong K. H. , Shim J. B. , Jeong Y. K. , Jang M.Y. , Jo Y. J. , Min S. G. , Chun J. Y. 2014 Effect of super- or sub-critical water treatment on physicochemical properties of pig skin. P 13-033 2014 Annual Meeting of Korean Society of food Sci. and Technol. Gwangju, Korea 164 - 165
Kim S. K. , Kim Y. T. , Byun H. G. , Park P. J. , Ito H. 2001 Purification and characterization of antioxidative peptides from bovine skin J. Biochem. Mol. Biol. 34 214 - 219
Kokini J. L. 1987 The physical basis of liquid food texture and texture-taste interactions J. Food Eng. 6 51 - 81    DOI : 10.1016/0260-8774(87)90021-5
Kus S. , Altan A. , Kaya A. 2005 Rheological behavior and time-dependent characterization of ice cream mix with different salep content J. Texture Stud. 36 273 - 288    DOI : 10.1111/j.1745-4603.2005.00016.x
Lee M. Y. , Choi Y. C. , Chun J. Y. , Min S. G. , Hong G.P. 2013 Effects of high pressure/high temperature processing on the recovery and characteristics of porcine placenta hydrolysayes Korean J. Food Sci. An. 33 474 - 480    DOI : 10.5851/kosfa.2013.33.4.474
Marshall R. T. , Goff H. D. , Hartel R. W. 2003 Ice cream 6th ed Kluwer Academic/Plenum Publishers NY 1 - 34
Morris E. R. , Stephen M. A. 1995 Food polysaccharides and their applications Marcel Dekker Inc NY Polysaccharide rheology and in-mouth perception 515 - 546
Muhr A. H. , Blanshard J. M. V. , Sheard S. J. 1986 Effects of polysaccharide stabilizers on the nucleation of ice J. Food Technol. 21 587 - 603
Nagarajan M. , Benjakul S. , Prodpran T. , Songtipya P. , Kishimura H. 2012 Characteristics and functional properties of gelatin from splendid squid (loligo formosana) skin as affected by extraction temperatures Food Hydrocolloid. 29 389 - 397    DOI : 10.1016/j.foodhyd.2012.04.001
Penninger J. M. L. , Kersten R. J. A. , Baur H. C. L. 2000 Hydrolysis of diphenylether in supercritical water: Effects of dissolved nacl J. Supercrit. Fluid. 17 215 - 226    DOI : 10.1016/S0896-8446(00)00046-2
Ravber M. , Knez Ž , Škerget M. 2015 Simultaneous extraction of oil- and water-soluble phase from sunflower seeds with subcritical water Food Chem. 166 316 - 323    DOI : 10.1016/j.foodchem.2014.06.025
Rincon F. , Leon de Pinto G. , Beltran O. 2006 Behavior of a mixture of Acacia glomerosa, Enterolobium cyclocarpum and Hymenaea courbaryl gums in ice cream preparation Food Sci. Technol. Int. 12 13 - 17    DOI : 10.1177/1082013206060445
Soukoulis C. , Chandrinos I. , Tzia C. 2008 Study of the functionality of selected hydrocolloids and their blends with κ-carrageenan on storage quality of vanilla ice cream Food Sci. Technol. 41 1816 - 1827
Soukoulis C. , Lebesi D. , Tzia C. 2009 Enrichment of ice cream with dietary fibre: effects on rheological properties, ice crystallisation and glass transition phenomena Food Chem. 115 665 - 671    DOI : 10.1016/j.foodchem.2008.12.070
Stanley N. I. , Taylor L. J. 1993 Rheological basis of oral characteristics of fluid and semi-solid foods: A review Acta. Psychol. 84 79 - 92    DOI : 10.1016/0001-6918(93)90074-2
Wang S. Y. , Damodaran S. 2009 Ice-structuring peptides derived from bovine collagen J. Agric. Food Chem. 57 5501 - 5509    DOI : 10.1021/jf900524y
Watchararuji K. , Goto M. , Sasaki M. , Shotiprunk A. 2008 Value-added subcritical water hydrolysate from rice bran and soybean meal Bioresour. Technol. 99 6207 - 6213    DOI : 10.1016/j.biortech.2007.12.021
Wildmoser H. , Scheiwiller J. , Windhab E. J. 2004 Impact of disperse microstructure on rheology and quality aspects of ice cream LWT-Food Sci. Technol. 37 881 - 891    DOI : 10.1016/j.lwt.2004.04.006
Zhu G. Y. , Zhu X. , Fan Q. , Wan X. L. 2011 Raman spectra of amino acids and their aqueous solutions Spectrochim. Acta, Part A 78 1187 - 1195    DOI : 10.1016/j.saa.2010.12.079